基于萘酰亚胺的硫化氢荧光探针的合成及光谱性能研究  

Synthesis and Spectral Properties of Hydrogen Sulfide Fluorescent Probe Based on Naphthalimide

作　　者：郭心灵 李静[2] 李玄 郭磊磊 赵丽平 侯旭锋[2] GUO Xin-ling;LI Jing;LI Xuan;GUO Lei-lei;ZHAO Li-ping;HOU Xu-feng(College of Biological and Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China;College of Chemistry and Chemical Engineering,Xuchang University,Xuchang 461000,China)

机构地区：[1]信阳农林学院生物与制药工程学院,河南信阳464000 [2]许昌学院化学化工学院,河南许昌461000

出　　处：《化学试剂》2020年第8期976-981,共6页Chemical Reagents

基　　金：河南省高校重点科研计划项目(19B150017);生物无机化学与药物湖北省重点实验室开放基金项目(BCMM201803);河南省高等学校达标创优基层教学组织建设项目(2018667);信阳农林学院专业综合改革试点建设项目(ZYZHGG201803)。

摘　　要：以6-羟基-萘酰亚胺和2-(2-吡啶基)二硫基苯甲酸为原料,并引入亲水性溶酶体靶向定位基团吗啉,设计合成一种新型萘酰亚胺硫化氢荧光探针,合成方法简单、产率较高、产物易于提纯。通过光学活性测试,探针对硫化氢的响应速度较快(5 min),在0~0.2μmol/L的范围内,荧光强度与硫化氢浓度呈现较好的线性关系(R2=0.9933),检出限低至6.272×10-8 mol/L,其在较宽的pH(5~9)范围内光学性质稳定,并可实现可视化检测和水样检测。研究结果表明,探针对硫化氢具有高效、高灵敏度、高选择性和定位检测,在硫化氢生理活性检测和病理分析检测方面具有潜在的应用前景。A novel naphthalimide fluorescent probe was synthesized based on 6-hydroxy-naphthalimide and 2-(2-pyridyl)dithiobenzoic acid,introducing morpholine,a hydrophilic lysosome targeting group.The synthesis method was simple and the productivity was high and the product was easily to be purified.Based on the optical activity test,the response speed of the probe to hydrogen sulfide was fast(5 min).In the range of 0~0.2μmol/L,fluorescence intensity and hydrogen sulfide concentration had a good linear relationship with the detection limit of 6.272×10-8 mol/L(R2=0.9933).Its optical property was stable in a wide range of pH(5~9),and it can realize visual detection and water sample detection.The results showed that the probe had high efficiency,high sensitivity,high selectivity for hydrogen sulfide and location detection,and had potential application in the detection of physiological activity and pathological analysis of hydrogen sulfide.

关 键 词：可视化检测 硫化氢 萘酰亚胺 荧光探针 

分 类 号：O656.3[理学—分析化学]