福建省某规模化猪场种公猪精液PRRSV检测及ORF7基因序列分析  

PRRSV Detection and ORF7 Gene Sequence Analysis of Boar Semen in a Large-scale Pig Farm in Fujian Province

作　　者：杨小燕 魏春华 戴爱玲 戴巍 刘建奎 YANG Xiao-yan;WEI Chun-hua;DAI Ai-ling;DAI Wei;LIU Jian-kui(College of Life Sciences of Longyan University,Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology,Fujian Engineering Research Center for Swine Disease Control and Prevention,Longyan 364000,China)

机构地区：[1]龙岩学院生命科学学院福建省家畜传染病防治与生物技术重点实验室福建省生猪疫病防控工程技术研究中心,福建龙岩364000

出　　处：《中国兽医杂志》2020年第3期57-60,共4页Chinese Journal of Veterinary Medicine

基　　金：福建省科技厅重大专项专题项目(2019NZ09005);福建省科技引导性项目(2018N0022);龙岩学院产学研项目(LC2017005);奇迈科技创新基金项目(XLQM006);龙岩学院博士启动金(0000090308)。

摘　　要：为了解福建省某规模化猪场种公猪猪繁殖与呼吸综合征病毒(PRRSV)的流行情况和流行特点。按照10%比例随机采集该场种公猪精液样本17份,通过RT-PCR方法对其进行PRRSV检测及ORF 7基因序列分析。结果表明,9份精液PRRSV呈阳性,样品阳性率为52.7%;遗传进化树分析表明,该猪场PRRSV ORF 7基因与QYYZ、GM2、FJFS等谱系3毒株亲缘关系较近,处于同一进化分支,而与VR2332、CH-1a、NADC30、JXA1和HuN4等亲缘关系较远,处于不同的进化分支,表明该毒株为近期新流行的谱系3的毒株。To investigate the prevalence and epidemic characteristics of porcine reproductive respiratory syndrome virus(PRRSV)in boar of a large-scale pig farm in Fujian province.The semen samples were randomly collected at a 10%ratio for PRRSV detection and ORF 7 gene sequence was analyzed by RT-PCR.The results showed that the PRRSV infection rate in the boar semen was 52.9%(9/17).Genetic phylogenetic tree analysis indicated that the PRRSV ORF 7 gene of this farm was closely related to QYYZ,GM2,FJFS(lineage 3 strains),but far from VR2332,CH-1a,NADC30,JXA1 and HuN4,indicating that the virus in this farm is belong to the lineage 3.

关 键 词：猪繁殖与呼吸综合征病毒 精液 ORF7基因 基因序列分析 

分 类 号：S852.651[农业科学—基础兽医学]