电针对大鼠心肌缺血再灌注时心肌电生理特性的影响  

Effect of electroacupuncture on electrophysiological characteristics of ventricular myocardium during myocardial ischemia-reperfusion in rats

作　　者：唐剑 刘艳秋 高鸿 任文鑫 黄岁岁 王贵龙 何幼芹 Tang Jian;Liu Yanqiu;Gao Hong;Ren Wenxin;Huang Suisui;Wang Guilong;He Youqin(School of Anesthesioloy,Guizhou Medical University,Guiyang 550004,China;Department of Anesthesiology,the Fourth People′s Hospital of Guiyang City,Guiyang 550004,China;the Third Affiliated Hospital of Guizhou Medical University,Duyun 558000,China)

机构地区：[1]贵州医科大学麻醉学院,贵阳550004 [2]贵阳市第四人民医院麻醉科,550004 [3]贵州医科大学第三附属医院,都匀558000

出　　处：《中华麻醉学杂志》2020年第4期433-436,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价电针对大鼠心肌缺血再灌注时心肌电生理特性的影响。方法清洁级健康成年雄性SD大鼠24只,体重280~320 g,采用随机数字表法分为3组(n=8):假手术组(SH组)、心肌缺血再灌注组(I/R组)和电针组(EA组)。采用结扎左冠状动脉前降支30 min再灌注30 min的方法制备心肌缺血再灌注损伤模型。IR+EA组于再灌注同时电针双侧前肢内关穴30 min。于结扎前(T0)、结扎30 min(T1)、再灌注30 min(T2)时记录HR和及左心室前壁外膜层心肌单相动作电位振幅(MAPA)、最大去极化速度(Vmax)、90%单相动作电位时程(MAPD90)。记录再灌注期间心律失常的发生情况和心律失常评分。结果与SH组比较,I/R组和EA组T1,2时HR减慢,MAPA和Vmax降低,MAPD90延长,室性早搏、室性心动过速和室颤的发生率、心律失常评分升高(P<0.05);与I/R组比较,EA组T2时HR增快,MAPA和Vmax升高,MAPD90缩短,室性心动过速和室颤的发生率、心律失常评分降低(P<0.05)。结论电针可加快心肌除极和缩短复极,从而降低大鼠再灌注心律失常发生。Objective To evaluate the effect of electroacupuncture(EA)on electrophysiological characteristics of ventricular myocardium during myocardial ischemia-reperfusion(I/R)in rats.Methods Twenty-four clean-grade healthy adult male Sprague-Dawley rats,weighing 280-320 g,were divided into 3 groups(n=8 each)using a random number table method:sham operation group(group SH),I/R group and group EA.The model of myocardial I/R injury was established by ligating the left anterior descending branch of coronary artery for 30 min followed by 30-min reperfusion in anesthetized rats.Bilateral Neiguan acupoints in forelimbs were stimulated for 30 min during the period of reperfusion in group EA.Heart rate(HR),monophasic action potential amplitude(MAPA),maximum depolarization rate(Vmax),and monophasic action potential duration at 90%repolarization(MAPD90)were recorded.The development of arrhythmias and arrhythmias score were recorded during reperfusion.Results Compared with group SH,HR was significantly decreased,MAPA and Vmax were decreased,MAPD90 was prolonged,and the incidence of ventricular premature beat,ventricular tachycardia and ventricular fibrillation and arrhythmias score were increased at T1,2 in I/R and EA groups(P<0.05).Compared with group I/R,HR was significantly increased,MAPA and Vmax were increased,MAPD90 was shortened,and the incidence of ventricular tachycardia and ventricular fibrillation and arrhythmias score were decreased at T2 in EA group(P<0.05).Conclusion EA can accelerate myocardial depolarization and shorten repolarization,thus decreasing the occurrence of reperfusion arrhythmia in rats.

关 键 词：电刺激疗法 心肌再灌注损伤 心律失常 心性 动作电位 

分 类 号：R245.97[医药卫生—针灸推拿学]