同型半胱氨酸致足细胞凋亡中FoxO1 DNA甲基化水平增高  

Increased FoxO1 DNA methylation level in homocysteine-induced podocyte apoptosis

作　　者：刘昆 谢琳[2,3,4] 曹军[1,2,3] 丁宁 徐灵博[2,3,4] 马胜超[2,3,4] 李桂忠[2,3,4] 姜怡邓 卢冠军[2,3,5] Liu Kun;Xie Lin;Cao Jun;Ding Ning;Xu Lingbo;Ma Shengchao;Li Guizhong;Jiang Yideng;Lu Guanjun(Clinical Medical College,Yinchuan 750004,Ningxia Hui Autonomous Region,China;Key Laboratory of Metabolic Cardiovascular Disease Research of National Health Commission,Yinchuan 750004,Ningxia Hui Autonomous Region,China;Ningxia Key Laboratory of Vascular Injury and Repair Research,Yinchuan 750004,Ningxia Hui Autonomous Region,China;Basic Medical College,,Yinchuan 750004,Ningxia Hui Autonomous Region,China;General Hospital of Ningxia Medical University,Yinchuan 750004,Ningxia Hui Autonomous Region,China)

机构地区：[1]宁夏医科大学临床医学院,宁夏回族自治区银川市750004 [2]宁夏医科大学国家卫生健康委代谢性心血管疾病研究重点实验室,宁夏回族自治区银川市750004 [3]宁夏医科大学宁夏血管损伤与修复研究重点实验室,宁夏回族自治区银川市750004 [4]宁夏医科大学基础医学院,宁夏回族自治区银川市750004 [5]宁夏医科大学总医院,宁夏回族自治区银川市750004

出　　处：《中国组织工程研究》2021年第2期269-273,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81560120),项目负责人:卢冠军。

摘　　要：背景:同型半胱氨酸增多会引起肾损伤并导致足细胞凋亡,但是其具体机制还尚不清楚。目的:探讨叉头框转录因子O1(forkhead box O,FoxO1)及其DNA甲基化在同型半胱氨酸致足细胞凋亡中的作用。方法:体外培养小鼠肾脏足细胞(MPC-5),将其分为对照组(0μmol/L同型半胱氨酸)和同型半胱氨酸组(80μmol/L同型半胱氨酸)。干预细胞48 h后,采用免疫荧光技术检验足细胞凋亡相关蛋白Bax、caspase12和Bcl-2的表达情况;采用实时荧光定量PCR(qRT-PCR)检测FoxO1 mRNA水平;采用Western blot检测FoxO1和DNMT1蛋白表达水平;采用巢式降落式特异性PCR(nMS-PCR)测验FoxO1的DNA甲基化水平。结果与结论:①与对照组相比,同型半胱氨酸组足细胞中Bax和caspase12表达明显增高,Bcl-2表达明显降低;②FoxO1的mRNA和蛋白表达水平明显降低(P<0.01);③与对照组相比,同型半胱氨酸组FoxO1 DNA甲基化水平明显升高(P<0.01),同型半胱氨酸组足细胞中DNMT1蛋白表达明显增高(P<0.01);④结果表明:FoxO1 DNA高甲基化在同型半胱氨酸致足细胞凋亡中作用显著,而DNMT1参与同型半胱氨酸诱导的足细胞凋亡过程。BACKGROUND:The increase of homocysteine can lead to renal injury and podocyte apoptosis,but the specific mechanism is not clear.OBJECTIVE:To investigate the effect of Forkhead box O1(FoxO1)and its DNA methylation in podocyte apoptosis induced by homocysteine.METHODS:Mouse renal podocytes(MPC-5)were cultured in vitro and divided into control group(0μmol/L homocysteine)and homocysteine group(80μmol/L homocysteine).After 48 hours of intervention,the expression of podocyte apoptosis-related proteins Bax,caspase12 and Bcl-2 was detected by immunofluorescence technique;the expression level of FoxO1 mRNA was detected by real-time fluorescence quantitative PCR;the protein expression levels of FoxO1 and DNMT1 were detected by western blot;DNA methylation level of FoxO1 was detected by nested methylation-specific PCR.RESULTS AND CONCLUSION:Compared with the control group,the expression levels of Bax and caspase12 protein in podocytes of the homocysteine group were significantly increased,while the expression of Bcl-2 protein was significantly decreased.The expression levels of FoxO1 mRNA and protein were significantly decreased in the homocysteine group compared with the control group(P<0.01).At the same time,the methylation level of FoxO1 DNA in the homocysteine group was significantly higher than that in the control group(P<0.01),and the expression of DNMT1 protein in podocytes in the homocysteine group was significantly higher than that in the control group(P<0.01).To conclude,FoxO1 DNA hypermethylation plays a significant role in podocyte apoptosis induced by homocysteine,whereas DNMT1 participates in homocysteine-induced podocyte apoptosis.

关 键 词：肾 肾小球 肾损伤 同型半胱氨酸 足细胞 凋亡 因子 甲基化 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]