辅助性T淋巴细胞22和白细胞介素-22在幼年特发性关节炎中的表达及与炎性因子水平的相关性  

Expressions of helper T lymphocytes 22 and interleukin-22 in juvenile idiopathic arthritis and their correlation with levels of inflammatory cytokines

作　　者：冯淼[1] 朱佳[2] 何峰[1] 肖宗慧[1] 姚海兰[1] Feng Miao;Zhu Jia;He Feng;Xiao Zonghui;Yao Hailan(Department of Biochemistry and Immunology,Capital Institute of Pediatrics,Beijing 100020,China;Department of Rheumatology,the Affiliated Children′s Hospital of Capital Institute of Pediatrics,Beijing 100020,China)

机构地区：[1]首都儿科研究所生化免疫研究室,北京100020 [2]首都儿科研究所附属儿童医院风湿免疫科,北京100020

出　　处：《中华实用儿科临床杂志》2020年第15期1169-1172,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(81601399)。

摘　　要：目的探讨幼年特发性关节炎(JIA)患儿外周血中辅助性T淋巴细胞(Th)22及其功能分子白细胞介素(IL)-22的表达水平,分析其与JIA相关炎性因子水平的相关性。方法选取2018年11月至2019年12月在首都儿科研究所附属儿童医院风湿免疫科住院的JIA患儿30例为JIA组,选取同期来首都儿科研究所附属儿童医院体检的同年龄健康儿童12例为健康对照组。采用流式细胞术检测2组研究对象外周血中Th22细胞比例,酶联免疫吸附实验检测血浆中细胞因子IL-22、IL-6、肿瘤坏死因子-α(TNF-α)、IL-17、IL-10的表达水平。比较JIA组和对照组Th22细胞比例和IL-22表达水平的差异,对Th22细胞比例和IL-22表达水平与炎性因子IL-6、TNF-α、IL-17、IL-10水平进行相关性分析。结果 JIA组外周血中Th22细胞比例[(0.94±0.26)%]明显高于对照组[(0.46±0.29)%],差异有统计学意义(t=2.227,P<0.05);JIA组血浆中IL-22水平[(185.2±11.93) ng/L]显著高于健康对照组[(114.7±6.29) ng/L],差异有统计学意义(t=3.632,P<0.001)。JIA组Th22细胞比例与血浆IL-6(r=0.501,P<0.01)、IL-17(r=0.686,P<0.001)、IL-10(r=0.609,P<0.001)水平均呈正相关,血浆IL-22水平与IL-6(r=0.573,P<0.01)、IL-17(r=0.445,P<0.01)、IL-10(r=0.284,P<0.001)水平均呈正相关,JIA组Th22细胞比例及血浆IL-22水平与血浆TNF-α水平均无明显相关性。结论 JIA患儿外周血中Th22细胞比例及其功能分子IL-22水平显著增高,与炎性因子具有一定相关性,可能参与了JIA疾病的发生和进展。Objective To investigate the proportion of helper T lymphocytes 22(Th22)and levels of interleukin(IL)-22 in peripheral blood of children with juvenile idiopathic arthritis(JIA),and analyze their relevance with JIA-related inflammatory cytokines.Methods A total of 30 children with JIA who received treatment at the Department of Rheumatology,the Affiliated Children′s of Capital Institute of Pediatrics from November 2018 to December 2019 were enrolled as JIA group,and 12 healthy children at the same age were selected as healthy control group.The percen-tage of Th22 cells in peripheral blood was detected using flow cytometry.Concentrations of IL-22,IL-6,tumor necrosis factor(TNF-α),IL-17 and IL-10 were measured by enzyme-linked immunosorbent assay.Statistical analysis of the relevance of Th22 cells,IL-22 levels and inflammatory cytokines levels of IL-6,TNF-α,IL-17 and IL-10 in JIA were performed by Pearson test.Results The proportion of Th22 cells in peripheral blood of patients in JIA group[(0.94±0.26)%]was higher than that of the healthy control group[(0.46±0.29)%],and the difference was statistically significant(t=2.227,P<0.05).Plasma level of IL-22 of patients in JIA group[(185.2±11.93)ng/L]was significantly higher than that of healthy control group[(114.7±6.29)ng/L],and the difference was statistically significant(t=3.632,P<0.001).The proportion of Th22 cells and the levels of plasma IL-22 in JIA patients were positively correlated with plasma levels of IL-6(Th22:r=0.501,IL-22:r=0.573,all P<0.01),IL-17(Th22:r=0.686,P<0.001;IL-22:r=0.445,P<0.01)and IL-10(Th22:r=0.609,IL-22:r=0.284,all P<0.001).There was no relationship for Th22 cells and plasma levels of IL-22 with TNF-α.Conclusions The proportion of Th22 cells and plasma levels of IL-22 significantly increase in peripheral blood of JIA patients and correlated with JIA-related inflammatory cytokines,which may play a potential role in the pathogenesis of JIA disease.

关 键 词：幼年特发性关节炎 TH22细胞 白细胞介素-22 炎性因子 

分 类 号：R725.9[医药卫生—儿科]