异基因造血干细胞移植后免疫细胞重建动力学模型的构建  

Establishing kinetic models of immune cell reconstitution after allogeneic hematopoietic stem cell transplantation

作　　者：苏龙[1] 高素君[1] 刘晓亮[1] 宋飞[1] 谭业辉[1] 刘宇[1] 赵杨祉[1] 孙京男[1] 韩薇[1] 戎月 李薇[1] Su Long;Gao Sujun;Liu Xiaoliang;Song Fei;Tan Yehui;Liu Yu;Zhao Yangzhi;Sun Jingnan;Han Wei;Rong Yue;Li Wei(Department of Hematology,the First Hospital of Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院肿瘤中心血液科,长春130021

出　　处：《中华器官移植杂志》2020年第6期351-357,共7页Chinese Journal of Organ Transplantation

基　　金：吉林大学第一医院临床研究培育基金(LCFYJJ2017005)。

摘　　要：目的采用数学函数模型研究异基因造血干细胞移植(allo-HSCT)后免疫细胞重建规律。方法回顾分析2011年6月至2015年5月吉林大学第一医院肿瘤中心血液科行allo-HSCT的65例恶性血液病受者,于移植后14 d、28 d、42 d、2个月、3个月、6个月、9个月、12个月、18个月、24个月监测免疫细胞亚群数量与百分比,包括CD3+T淋巴细胞、CD4+辅助性T淋巴细胞、CD8+效应T淋巴细胞、调节性T淋巴细胞、CD19+B淋巴细胞、CD3-CD56+NK细胞及CD3+CD56+NK样T淋巴细胞。通过曲线模型估算,建立曲线模型及相应的数学方程并验证。结果移植后免疫细胞百分比变化规律全部符合立方曲线模型。免疫细胞绝对值中除CD3+T淋巴细胞、CD8+T淋巴细胞与NK细胞外,余均符合立方曲线模型。CD8+T淋巴细胞与NK细胞移植后重建动力学模型与疾病复发有关。结论allo-HSCT后免疫细胞重建均符合一定的数学函数模型,这可能为深入研究allo-HSCT后免疫重建提供新的策略。Objective To explore immune cell reconstitution after allogeneic hematopoietic stem cell transplantation(allo-HSCT)by using mathematical function models.Methods From June 2011 to May 2015,65 patients with malignant hematological disorders were retrospectively analyzed.Immune cell frequencies and absolute counts were detected at day 14/28/42 and month 2/3/6/9/12/18/24 post-allo-HSCT.The immune cells included CD3+T,CD4+helper T,CD8+effector T,regulatory T,CD19+B,CD3-CD56+NK and CD3+CD56+NKT.Kinetic curve models and mathematical equations were established by utilizing curve model estimation.Results Cubic curve models were observed for the changes of immune cell frequencies.Except for CD3+T,CD8+T and NK cells,the changes of absolute counts of immune cells conformed to cubic curve models.The reconstructed kinetic models of CD8+T and NK cells after allo-HSCT were associated with relapse.Conclusions Immune cell reconstitution after allo-HSCT conforms to certain mathematical function curve models.It may provide a new strategy for in-depth studies of immune reconstitution after allo-HSCT.

关 键 词：造血干细胞移植 恶性血液病 免疫 

分 类 号：R457.7[医药卫生—治疗学]