寒泊羊群体中BMPR-IB基因多态性及其对胎产羔数的影响  

Polymorphism of the BMPR-IB gene in Hanper mutton sheep population and its effect on litter size

作　　者：马晓菲 陈晓勇[2] 刘爱菊[1] 韩红叶 孙欢 李清艳 敦伟涛[2] 孙树春[1,4] 田树军 MA Xiaofei;CHEN Xiaoyong;LIU Aiju;HAN Hongye;SUN Huan;LI Qingyan;DUN Weitao;SUN Shuchun;TIAN Shujun(College of Animal Science and Technology of Hebei Agricultural University,Baoding 071000,China;Hebei Province Institute of Animal Husbandry,Baoding 071000,China;Agriculture Science and Education Center of Hebei Agricultural University,Baoding 071000,China;Hebei Province Cattle and Sheep Embryo Engineering Technology Research Center,Baoding 071000,China)

机构地区：[1]河北农业大学动物科技学院,河北保定071000 [2]河北省畜牧兽医研究所,河北保定071000 [3]河北农业大学科教兴农中心,河北保定071000 [4]河北省牛羊胚胎工程技术研究中心,河北保定071000

出　　处：《畜牧与兽医》2020年第8期6-11,共6页Animal Husbandry & Veterinary Medicine

基　　金：河北省羊产业技术体系(HBCT2018140202,HBCT2018140403);河北省重点研发计划项目(20326349D)。

摘　　要：旨在揭示寒泊羊群体BMPR-IB基因位点发生突变的多态性,以及其不同基因类型对母羊胎产羔数的影响。利用PCR-RFLP方法判定寒泊羊个体BMPR-IB基因位点的基因类型,统计不同基因型在寒泊羊种群体中所占比例,并运用相关性分析以及多元线性回归分析法,探究不同基因型和不同胎次对母羊胎产羔数的影响。结果:BMPR-IB基因在寒泊羊群体中有BB、B+和++3种基因类型,其基因型频率分别为16.66%、33.34%和50.00%。初产母羊BB、B+和++基因型的平均胎产羔数分别为1.87、1.73和1.32只;经产母羊BB、B+和++基因型的平均胎产羔数分别为2.30、2.12和1.54只。母羊所携带的B基因的数量、胎次都与胎产羔数呈显著正相关,其中B基因的影响最大。结论:BMPR-IB基因在寒泊羊种群中存在BB、B+和++3种基因类型,其基因型频率分别为16.66%、33.34%和50.00%,B基因对胎产羔数具有正效应。This research was to reveal the polymorphism of the BMPR-IB gene in Hanper mutton sheep population and the effects of different gene types on the litter size in the ewe.The gene type of BMPR-IB of the Hanper mutton sheep was determined using PCR-RFLP.The proportion of different genotypes in the Hanper mutton sheep population was calculated.Correlation analysis and multiple linear regression analysis were performed to identify the effects of different genotypes and different parities on the litter size in the ewe.The results showed that the BMPR-IB gene had three genotypes of BB,B+and++in the Hanper mutton sheep population,and the genotype frequencies were 16.66%,33.34%and 50.00%,respectively.For primiparous ewes,the average litter sizes of BB,B+and++genotypes were 1.87,1.73 and 1.32,respectively.For multiparous ewes,the average litter sizes of BB,B+and++genotypes were 2.30,2.12 and 1.54,respectively.The number of B genes carried by the ewes and the parity were significantly positively correlated with the litter size in the ewe,between which the B gene had the greater effect.To conclude,the BMPR-IB gene had three genotypes of BB,B+and++in the population of Hanper mutton sheep,and the genotype frequencies were 16.66%,33.34%and 50.00%,respectively.The B gene had a positive effect on litter size.

关 键 词：寒泊羊 BMPR-IB基因 产羔数 

分 类 号：S826[农业科学—畜牧学]