不同种质资源银柴胡抗旱性研究  

Study on drought resistance of different germplasm resources of Stellaria dichotoma

作　　者：叶旭波 马玲芳 李阳[1] 代晓华[1] YE Xubo;MA Lingfang;LI Yang;DAI Xiaohua(School of Agriculture,Ningxia Univesity,Yinchuan 750000,China)

机构地区：[1]宁夏大学农学院,银川750000

出　　处：《植物生理学报》2020年第6期1295-1304,共10页Plant Physiology Journal

基　　金：宁夏回族自治区育种专项(2014-327);宁夏回族自治区重点研发计划(重点)项目(2019BBF02029)。

摘　　要：银柴胡(Stellaria dichotoma)是干旱地区重要的药材种类,由于资源匮乏,人工种植已成为主要的药材供给途径,但在种植中出现各种植区种子相互引种,种源混乱不清,种子杂等诸多问题。为筛选出适宜宁夏干旱区人工种植的优质银柴胡种质资源,本研究通过对收集的不同产地银柴胡种子进行盆栽试验,在苗期以15%PEG溶液浇灌模拟干旱胁迫做处理,测定胁迫3d后银柴胡植株的生理指标,利用隶属函数数值法综合评价不同种质资源银柴胡的抗旱性。结果表明,不同种质资源的抗旱性强弱顺序为:11>2>13>12>9>14>10>6>8>5>1>3>7>4。11号种质资源的过氧化物酶活性与脯氨酸显著高于2号种质资源和13号种质资源,综合生理指标显示11号种质资源的抗旱性优于其他13种银柴胡种质资源,其次为2号和13号种质资源。Stellaria dichotoma is a crucial traditional Chinese medicine material in arid areas.Artificial cultivation of this materials is often observed as satisfying with supply route due to scarcity of resources.However,there exists serious problems during the artificial cultivation of Stellaria dichotoma such as the interaction introduction in different growing areas,confusion of germplasm,clutter of seed and etc.In order to screen out the germplasm resources of high quality S.dichotoma suitable for artificial planting in arid area of Ningxia,this article evaluated the potted cultivation experiment results of the Stellaria dichotoma seeds from different origins.In the potted cultivation experiment,the seeds were watered with 15%PEG solution at the seedling stage to simulate the drought stress status,and three days after which measured the physiological indexes of S.dichotoma seeds.Then,analysis the drought resistance of 14 species of S.dichotoma comprehensively based on membership function.The experimental result shows the drought resistance order of different germplasm resources,from strong to weak,were 11>2>13>12>9>14>10>6>8>5>1>3>7>4.The activity of peroxidase and proline of germplasm resource 11 was significantly higher than that of germplasm resource 2 and germplasm resource 13.The comprehensive physiological indexes showed that the drought resistance of germplasm resource 11 was better than that of 13 other germplasm resources,followed by germplasm resource 2 and germplasm resource 13.

关 键 词：银柴胡 干旱胁迫 抗旱性 

分 类 号：S567.79[农业科学—中草药栽培]