MiR-155在老年原发免疫性血小板减少症患者预后评估中的价值  

Value of MiR-155 in Prognostic Evaluation of Elderly Patients with Primary Immune Thrombocytopenia

作　　者：何瑛[1] 魏学花 糟秀梅 HE Ying;WEI Xue-Hua;ZAO Xiu-Mei(Department of Hematology,The First People′s Hospital of Yinchuan,Yinchuan 750001,Ningxia Hui Autonomous Region,China)

机构地区：[1]银川市第一人民医院血液内科,宁夏银川750001

出　　处：《中国实验血液学杂志》2020年第4期1338-1343,共6页Journal of Experimental Hematology

摘　　要：目的:探讨外周血单个核细胞(PBMNC)中has-microRNA-155(miR-155)在老年原发免疫性血小板减少症(PITP)患者预后的评估价值。方法:选取我院诊治的PITP老年患者130例和健康志愿者60例,采用RT-PCR法检测PBMNC中miR-155相对表达水平。采用非条件Logistic多因素回归分析miR-155表达与PITP预后的相关性,采用Kaplan-Meier进一步分析miR-155和PITP复发的关系。结果:PITP患者PBMNC中miR-155相对表达水平显著高于健康志愿者,并随疾病严重程度而显著升高(P<0.05)。miR-155低表达组PITP老年患者的糖皮质激素治疗总有效率显著高于miR-155高表达组(96.92%vs 72.31%)。多因素分析显示,miR-155是PITP患者的独立危险因素,同时miR-155高表达的老年患者复发风险更高。结论:PBMNC中miR-155高表达的PITP老年患者具有较差的预后和更高的复发风险。Objective:To explore the value of has-microRNA-155(miR-155)in peripheral blood mononuclear cells(PBMNC)in prognostic evaluation of elderly patients with primary immune thrombocytopenia(PITP).Methods:One hundred and thirty elderly PITP patients and 60 healthy volunteers in our hospital were selected.The relative expression level of miR-155 in PBMNC was detected by RT-PCR.Unconditional logistic multivariate regression analysis was used to analyze the relationship between miR-155 expression and prognosis of PITP patients,and Kaplan-Meier was further used to analyze the relationship between miR-155 and PITP recurrence.Results:The relative expression level of miR-155 in PBMNC of elderly PITP patients was significantly higher than that in healthy volunteers,and increased significantly with the severity of the disease(P<0.05).The overall effective rate of elderly PITP patients with miR-155 low-expression was significantly higher than that in the patients with miR-155 high-expression(96.92%vs 72.31%)by after treatment with glucocorticoid.Multivariate analysis showed that miR-155 was an independent risk factor for PITP patients.Elderly patients with high expression of miR-155 showed a higher risk of recurrence.Conclusion:miR-155 in PBMNC has a high accuracy for PITP diagnosis,and the elderly patients with high level of miR-155 show a poor prognosis and a higher risk of recurrence.

关 键 词：外周血单个核细胞 原发免疫性血小板减少症 has-microRNA-155 预后 复发 

分 类 号：R558.2[医药卫生—血液循环系统疾病]