HPLC法测定黄连中3种原小檗碱型生物碱的研究  

Determinations of Three Protoberberine Alkaloids in Rhizoma coptidis by High Performance Liquid Chromatography Method

作　　者：刘娜 管淑玉[2] 徐鹏[2] 杨然存 于阿娟[3] LIU Na;GUAN Shuyu;XU Peng;YANG Rancun;YU Ajuan(Henan Provincial Institute of Product Quality Supervision and Inspection,Zhengzhou 450001,China;National Center for Quality Supervision and Inspection of Building Decoration Materials,Zhengzhou 450001,China;College of Chemistry,Zhengzhou University,Zhengzhou 450002,China)

机构地区：[1]河南省产品质量监督检验院,郑州450001 [2]国家建筑装修材料质量监督检验中心,郑州450001 [3]郑州大学化学学院,郑州450002

出　　处：《河南科学》2020年第7期1057-1061,共5页Henan Science

基　　金：国家自然科学基金(21675144)。

摘　　要：建立了一种同时测定黄连中3种原小檗碱型生物碱的高效液相色谱法,该方法可以使黄连中的小檗碱、巴马汀、药根碱得以分离和检测.采用Agilent Zorbax C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-20 mmol/L磷酸二氢钾缓冲溶液(pH 3.0,含0.2%三乙胺)梯度洗脱,柱温25℃,流速1 mL/min,在波长为265 nm处检测.结果表明,各组分线性关系良好,相关系数R为0.999 5~0.999 6,加标回收率在92.9%~105.9%,检出限0.1~0.45μg/mL.A method based on high-performance liquid chromatography coupled with diode array detection(HPLCDAD)has been developed for simultaneous determination of three protoberberine akaloids(berberine,palmatine and jatrorrhizine)in Rhizoma coptidis. The analysis was performed on a Zorbax SB C18 column with gradient elution of water(0.02 mol·L-1 sodium dihydrogen phosphate,0.2% triethylamine,adjusted to p H 3 with phosphoric acid)and acetonitrile. The column temperature was 25 ℃,flow rate was 1 m L/min,and UV detection was set at 265 nm. All calibration curves showed good linearity with correlation coefficients 0.999 5-0.999 6 and spiked recovery were92.9%-105.9%. Limit of detection was in the range of 0.1-0.45 μg/mL. The results reveal that the established method is suitable for the determinations of berberine,palmatine and jatrorrhizine in R. coptidis.

关 键 词：黄连 原小檗碱型生物碱 小檗碱 巴马汀 药根碱 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]