玉米高频单倍体诱导系选育研究  

Study on Breeding the High Frequency Haploid Inducer in Maize

作　　者：段民孝[1] 刘新香[1] 邢锦丰[1] 张华生[1] 王元东[1] 宋伟[1] 张雪原[1] 杨海涛[1] 张春原[1] 吴珊珊 赵久然[1] DUAN Min-xiao;LIU Xin-xiang;XING Jin-feng;ZHANG Hua-sheng;WANG Yuan-dong;SONG Wei;ZHANG Xue-yuan;YANG Hai-tao;ZHANG Chun-yuan;WU Shan-shan;ZHAO Jiu-ran(Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding,Beijing 100097,China)

机构地区：[1]北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室,北京100097

出　　处：《玉米科学》2020年第4期8-14,共7页Journal of Maize Sciences

基　　金：国家重点研发计划项目(2016YFD0102003、2016YFD0101204);北京市农林科学院科技创新能力建设专项“玉米种质资源收集、评价与创新”(KJCX20170112)。

摘　　要：以3个杂交种京科968、先玉335、京科528及4个自交系京724、京92、京X005、京17为测验种,研究新选育单倍体诱导系的诱导率情况。结果表明,利用子粒颜色标记鉴别单倍体子粒存在误差,影响鉴别精度,通过田间种植进一步校正获得实际诱导率。同一诱导系不同单株的单倍体诱导率、不同测验种之间的单倍体诱导率均有差异。分析78份诱导系的140个穗系的282个测交组合,21份诱导系的25个测交组合的实际诱导率超过15.0%,最高达到25.3%。综合筛选出12份单倍体诱导系用于进一步研究。The induction rates of 80 new haploid induction lines were studied with three hybrids Jingke968,Xianyu335,Jingke528 and four inbred lines Jing724,Jing92,Jing X005 and Jing17 as test species.The results showed that there were errors in the identification of haploid kernel by means of grain color markers,which affected the identification accuracy,and the actual induction rate was obtained by the further correction in field planting.The haploid induction rates of different plants in the same inducer line and among different test species were different.Two hundred eighty-two test crosses combinations of 140 individual plants from 78 induced lines were analyzed.Among them,the actual induction rate of 25 test cross combinations of 21 induced lines was more than15.0%,and the highest induction rate was 25.3%.Twelve haploid induction lines were screened for further study.

关 键 词：玉米 单倍体育种 单倍体诱导系 单倍体诱导率 测验种 

分 类 号：S513.031[农业科学—作物学]