槐定碱对大鼠局灶性脑缺血损伤的保护作用  

Protective effects of sophoridine on focal cerebral ischemia injury in rats

作　　者：薛玉梅[1] 李晓兵[2] 薛占苍[1] 曹献国 李献军[1] XUE Yu-mei;LI Xiao-bing;XUE Zhan-cang;CAO Xian-guo;LI Xian-jun(Department of Neurology,The First Hospital of Shijiazhuang,Shijiazhuang 050011,Hebei Province,China;b.Department of Pharmacy,The First Hospital of Shijiazhuang,Shijiazhuang 050011,Hebei Province,China)

机构地区：[1]石家庄市第一医院神经内科,河北石家庄050011 [2]石家庄市第一医院药学部,河北石家庄050011

出　　处：《中国临床药理学杂志》2020年第15期2250-2253,共4页The Chinese Journal of Clinical Pharmacology

基　　金：石家庄市科学技术研究与发展指导计划课题资助项目(161460713)。

摘　　要：目的探索槐定碱对局灶性脑缺血损伤大鼠氧化应激和血清炎症因子水平的影响。方法按照体重将大鼠随机分成假手术组、对照组和低、中、高剂量实验组。低、中、高剂量实验组分别给予腹腔注射槐定碱2.5,5.0和10.0 mg·kg^-1·d^-1;假手术组和对照组均给予腹腔注射等体积0.9%NaCl,qd。5组大鼠均连续给药5 d。末次给药后,除假手术组外,其余各组用线栓法制作大鼠右侧大脑中动脉闭塞模型。用比色法检测大鼠血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)的含量,用酶联免疫吸附实验法检测大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的含量。结果造模24 h后,对照组和低、高剂量实验组的SOD分别为(123.53±13.51),(137.88±13.22)和(145.67±11.85)U·mL^-1,CAT分别为(4.60±1.04),(5.78±1.74)和(7.39±1.03)U·mL^-1,MDA分别为(11.02±1.12),(8.61±1.14)和(6.80±1.33)nmol·mL^-1,TNF-α分别为(172.65±9.67),(141.74±16.07)和(127.22±16.23)pg·mL^-1,IL^-1β分别为(121.52±14.87),(108.81±9.01)和(85.84±13.33)pg·mL^-1,IL-6分别为(673.69±96.43),(623.93±96.69)和(416.39±88.85)pg·mL^-1。高剂量实验组的上述指标与对照组相比,差异均有统计学意义(P<0.01,P<0.05);低剂量实验组的SOD、MDA、TNF-α和IL^-1β与对照组相比,差异均有统计学意义(P<0.01,P<0.05)。结论槐定碱对大鼠脑缺血损伤具有神经保护作用,其可以通过影响脑缺血大鼠的氧化应激和炎症反应,从而干预脑缺血损伤。Objective To explore the effects of sophoridine on oxidative stress and serum inflammatory factors in rats with focal cerebral ischemia.Methods Rats were randomly divided into sham operation,control,experimental-L,experimental-M and experimental-H groups according to the weight.Experimental-L,-M and-H groups received intraperitoneal injection of sophoridine 2.5,5.0 and 10.0 mg·kg^-1·d^-1 for 5 days,respectively.Middle cerebral artery occlusion model was established immediately by suture method in each group except sham operation group after the last administration.The contents of superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)in rat serum were measured by colorimetry,serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 were measured by enzyme-linked immunosorbent assay.Results Twenty-four hours after cerebral ischemia,the SOD contents in control and experimental-L/-H groups were(123.53±13.51),(137.88±13.22)and(145.67±11.85)U·mL^-1,the CAT contents were(4.60±1.04),(5.78±1.74)and(7.39±1.03)U·mL^-1,the MDA contents were(11.02±1.12),(8.61±1.14)and(6.80±1.33)nmol·mL^-1,the TNF-α contents were(172.65±9.67),(141.74±16.07)and(127.22±16.23)pg·mL^-1,the IL-1β levels were( 121. 52 ± 14. 87),( 108. 81 ± 9. 01) and( 85. 84 ± 13. 33)pg·mL^-1, the IL-6 levels were(673.69±96.43),(623.93±96.69)and(416.39±88.85)pg·mL^-1,respectively.There were significant differences in the above-mentioned indexes between experimental-H group and control group(P<0.01,P<0.05).And there were significant differences in SOD,MDA,TNF-α and IL-1β between experimental-L group and control group(P<0.01,P<0.05).Conclusion Sophoridine has neuroprotective effects on focal cerebral ischemia injury by affecting oxidative stress and inflammatory reaction in rats.

关 键 词：槐定碱 局灶性脑缺血 抗氧化酶 肿瘤坏死因子-α 白细胞介素-1Β 白细胞介素-6 

分 类 号：R28[医药卫生—中药学]