高效液相色谱串联质谱法测定人血浆中尼美舒利的浓度  

Determination of nimesulide in human plasma by high performance liquid chromatography-tandem mass spectrometry

作　　者：杜萍[1] 李鹏飞[1] 刘洪川[1] 刘丽宏[1] DU Ping;LI Peng-fei;LIU Hong-chuan;LIU Li-hong(Department of Pharmacy,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]首都医科大学附属北京朝阳医院药事部,北京100020

出　　处：《中国临床药理学杂志》2020年第15期2310-2312,2316,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金青年基金资助项目(81703611);北京市医院管理中心“青苗”计划专项经费资助项目(QML20180305)。

摘　　要：目的建立一种测定人血浆中尼美舒利浓度的高效液相色谱串联质谱方法。方法用Waters XBridge C18(2.1 mm×50.0 mm,3.5μm)色谱柱,流动相为含0.01%甲酸的乙腈和水,流速为0.45 mL·min^-1,梯度洗脱,柱温为室温,进样时间为5 min。内标为氢氯噻嗪,负离子检测模式。考察该方法的专属性、标准曲线与定量下限、精密度与准确度、基质效应。结果人血浆中尼美舒利在1~2000 ng·mL^-1内线性关系良好(r=0.9975),精密度和准确度均≤±15%,提取回收率在94.70%~110.91%,且不存在明显的基质效应。结论本方法简便、快速、准确,适用于人血浆中尼美舒利浓度的分析。Objective To establish a high performance liquid chromatography-tandem mass spectrometry method for the analysis of nimesulide in human plasma.Methods Water XBridge C18(2.1 mm×50.0 mm,3.5μm)column was used,the mobile phase was acetonitrile and water containing 0.01% formic acid,the flow rate was 0.45 mL·min^-1.The gradient elution mode was utilized,and the column temperature was set to room temperature.The whole analytical time was 5 min.The internal standard was hydrochlorothiazide and negative ion detection mode was used.Specificity,calibration curve,lower limit of quantification,precision and accuracy,and matrix effects were all investigated.Results The linear range was 1-2000 ng·mL^-1 with correlation coefficient(r=0.9975).The accuracy and precision were all ≤±15%.Mean recovery were all within the range of 94.70%-110.91%.There was no obvious matrix effects.Conclusion This simple,rapid and accurate method is suitable for the analysis of nimesulide concentration in human plasma.

关 键 词：尼美舒利 高效液相色谱串联质谱法 血药浓度 人血浆 

分 类 号：R971.6[医药卫生—药品]