特应性皮炎小鼠模型中白细胞介素-21、CC趋化因子配体20的表达分析  

Expression and analysis of interleukin-21 and CC chemokine ligand 20 in atopic dermatitis mouse model

作　　者：王娟[1] 郑爱义[1] 鲍海平[1] 赵一锦[1] WANG Juan;ZHENG Ai-yi;BAO Hai-ping;ZHAO Yi-jin(Department of Dermatology,Medical College of Shanxi Datong University,Shanxi Province,Datong 037009,China)

机构地区：[1]山西大同大学医学院皮肤教研室,山西大同037009

出　　处：《中国当代医药》2020年第23期14-16,共3页China Modern Medicine

基　　金：山西大同大学青年科研基金项目(2017Q4)。

摘　　要：目的测定白细胞介素-21(IL-21)和CC趋化因子配体20(CCL20)在2,4-二硝基氯苯(DNCB)诱导的特应性皮炎小鼠模型中的表达情况,探讨二者在特应性皮炎发病中的作用。方法将12只BALB/c雌性小鼠,随机分为模型组和对照组,每组6只,使用DNCB构建小鼠特应性皮炎模型。造模成功后采用酶联免疫吸附法检测小鼠血清中IL-21和CCL20的水平。结果特应性皮炎模型组小鼠血清中IL-21和CCL20的表达水平均高于对照组,差异有统计学意义(P<0.05),并且二者表达成正相关(r=0.59,P<0.05)。结论IL-21和CCL20在特应性皮炎小鼠模型中表达升高,且成正相关,可能协同参与了特应性皮炎的发生发展。Objective To detect the expression of interleukin-21(IL-21)and CC chemokine ligand 20(CCL20)in 2,4-dinitrochlorobenzene(DNCB)induced mice models with atopic dermatitis and explore their roles in the pathogenesis of atopic dermatitis.Methods Twelve female BALB/c mice were randomly divided into the model group and control group(6 in each).Mice models with atopic dermatitis were established by DNCB.After successful modeling,the serum levels of IL-21 and CCL20 were detected by enzyme-linked immunosorbent assay(ELISA).Results The serum levels of IL-21 and CCL20 in the model group with atopic dermatitis were significantly higher than those in the control group(P<0.05).There was a positive correlation between the serum levels of IL-21 and CCL20 in the model group(r=0.59,P<0.05).Conclusions The expression of IL-21 and CCL20 in the mouse model of atopic dermatitis is increased and positively correlated,which may be synergistically involved in the occurrence and development of atopic dermatitis.

关 键 词：特应性皮炎 白细胞介素-21 CC趋化因子配体20 小鼠模型 

分 类 号：R751[医药卫生—皮肤病学与性病学]