1株灰黄霉素产生菌的诱变选育及其发酵条件优化  

Mutation Breeding of a Griseofulin-producing Strain and the Optimization of Its Fermentation Conditions

作　　者：罗秀针[1] 郑金华[1] 林燕燕[1] 张文华[1] LUO Xiu-zhen;ZHENG Jin-hua;LIN Yan-yan;ZHANG Wen-hua(Zhangzhou Health Vocational College, Zhangzhou, Fujian 363000, China)

机构地区：[1]漳州卫生职业学院,福建漳州363000

出　　处：《福建农业科技》2020年第6期12-16,共5页Fujian Agricultural Science and Technology

基　　金：漳州市科技局自然科技基金(ZZ2020J38)。

摘　　要：以灰黄霉素产生菌ji2为出发菌株经过紫外线与氯化锂诱变处理,选育出灰黄霉素效价高且发酵周期较短的菌株UL10080,其效价为91304μg·mL^-1,比出发菌株ji2提高了47.8%,通过连续传代培养,其遗传稳定性良好。对UL 10080发酵条件进行优化,优化后液体发酵培养基:大米粉150 g·L^-1,NaCl 8.0 g·L^-1,CaCO38.0 g·L^-1,黄豆饼粉1.0 g·L^-1,KH2PO46.0 g·L^-1,KCl 7.0 g·L^-1,NaNO31.0 g·L^-1,FeSO41.0 g·L^-1,pH自然;优化后的培养条件:培养40 h的种子液以12%的接种量接种,28℃下培养12 d,灰黄霉素的效价从未优化前的91304μg·mL^-1提高到124965μg·mL^-1,提高了36.9%,与出发菌株ji2相比,UL10080产灰黄霉素的效价提高了1.02倍,发酵时间上缩短了48 h。By taking the griseofulin-producing strain ji-2 as the original strain,the strain UL10080 with high potency of griseofulvin and short fermentation period was selected after the mutagenesis with ultraviolet ray and lithium chloride.The potency of the strain UL10080 was 91304μg·mL^-1,which was 47.8%higher than that of the original strain ji-2,and the genetic stability was good through the continuous subculture.The fermentation conditions of UL10080 were optimized,and the optimized liquid fermentation medium was as follows:rice flour 150 g·L^-1,NaCl 8.0 g·L^-1,CaCO38.0 g·L^-1,soybean powder 1.0 g·L^-1,KH2PO46.0 g·L^-1,KCl 7.0 g·L^-1,NaNO31.0 g·L^-1,FeSO41.0 g·L^-1,and pH was natural.The optimized culture conditions were as follows:the seed solution cultured for 40 h was inoculated at 12%dose,and cultured at 28℃for 12 d.The potency of griseofulvin was increased from 91304μg·mL^-1 before the optimization to 124965μg·mL^-1,with an increase of 36.9%.While compared with the original strain ji-2,the potency of griseofulvin produced by UL10080 increased by 1.02 times,and the fermentation time was shortened by 48 h.

关 键 词：灰黄霉素 诱变育种 效价 培养基 培养条件优化 

分 类 号：Q939.9[生物学—微生物学]