金连菊复方制剂对小鼠的急性毒性试验  

Acute Toxicity Test of Compound Preparation of Jinlianju on Mice

作　　者：黄全明 周剑辉 杨昭远 兰宗宝[4] 俸祥仁 HUANG Quan-ming;ZHOU Jian-hui;YANG Zhao-yuan;LAN Zong-bao;FENG Xiang-ren(Yongfu County Agriculture and Rural Bureau, Guilin, Guangxi 541800, China;Guilin AnimalHusbandry Station, Guilin, Guangxi 541000, China;Institute of Animal Health in Jiangnan Districtof Nanning, Nanning, Guangxi 530001, China;Institute of Agricultural Science and TechnologyInformation, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China;Guangxi Baipeng Livestock Breeding Station, Liuzhou, Guangxi 541502, China)

机构地区：[1]永福县农业农村局,广西桂林541800 [2]桂林市畜牧站,广西桂林541000 [3]南宁市江南区动物卫生监督所,广西南宁530001 [4]广西农业科学院农业科技信息研究所,广西南宁530007 [5]广西百朋种畜场,广西柳州541502

出　　处：《福建农业科技》2020年第6期17-21,共5页Fujian Agricultural Science and Technology

基　　金：广西科技基地和人才专项(2019AC24026);南宁市西乡塘区科学研究与技术开发计划项目(20810203)。

摘　　要：以健康昆明种小鼠为动物模型,一次性经口灌服不同剂量(10.00、7.76、6.03、4.68、3.63和2.82 g·kg^-1)的金连菊复方制剂,通过开展小鼠急性毒性试验评价其用药安全性。结果表明:灌服不同剂量金连菊复方制剂后,小鼠首先呈现安静、趴卧不动、腹式呼吸,随着时间推移部分小鼠出现被毛竖立、眼球突出、尾部血管发蓝等症状,严重者腹部急剧收缩、行动不稳、抽搐死亡,但至给药后第4 d小鼠毒性反应基本消失;小鼠死亡现象主要发生在给药后24 h内,且呈明显的量效关系;剖检死亡小鼠发现其肺脏充血或有出血点,同时伴有胸腔积液;试验结束后将剩余小鼠全部处死剖检,发现各处理组小鼠的内脏器官均无明显病理变化,与正常小鼠的内脏器官一致。通过改良寇氏计算公式得到金连菊复方制剂对小鼠的半数致死量(LD50)为6.299 g·kg^-1,其标准误差(Sx50)为0.0355,95%置信区间为7.394~5.367,属于实际无毒性物质。By taking the healthy Kunming mice as the animal model,the compound preparation of Jinlianju was given different doses(10.00,7.76,6.03,4.68,3.63 and 2.82 g·kg^-1)by oral administration at one time.The acute toxicity test of mice was carried out to evaluate the drug safety.The results showed that after filling different doses of the compound preparation of Jinlianju,the mice showed quiet,laying still and abdominal breathing at first;As time went on,some mice showed clothing hair erection,protruding eyes and blue blood vessels in the tail.In the severe cases,the abdomen contracted sharply,the movement was unstable,and the mice twitched and died,but the toxic reaction of the mice basically disappeared at the 4th day after medication.The death of mice mainly occurred within 24 h after medication,and showed an obvious dose-effect relationship.The dead mice were found to have congestion in the lungs or bleeding points,as well as pleural effusion.After the end of the test,all the remaining mice were sacrificed for autopsy,and it was found that the internal organs of the mice in each treatment group had no obvious pathological changes,which was consistent with the internal organs of normal mice.By using the Karber method,the LD50 and Sx50 of Jinlianju compound preparation for mice were obtained,being 6.299 g·kg^-1 and 0.0355,respectively.And the confidence interval of 95%was 7.394-5.367,indicating that the compound preparation of Jinlianju belonged to the actual non-toxic substance.

关 键 词：金连菊复方制剂 小鼠 急性毒性试验 半数致死量(LD50) 

分 类 号：S853.74[农业科学—临床兽医学]