未来软骨修复的热点方向:层粘连蛋白促进干细胞增殖  

Future hot spots for cartilage repair:laminin promotes stem cell proliferation

作　　者：张晓波 张杰[2] 孙钰[3] Zhang Xiaobo;Zhang Jie;Sun Yu(Dalian Medical University,Dalian 116044,Liaoning Province,China;The Second Xiangya Hospital of Central South University,Changsha 410011,Hunan Province,China;Northern Jiangsu People’s Hospital,Yangzhou 225001,Jiangsu Province,China)

机构地区：[1]大连医科大学,辽宁省大连市116044 [2]中南大学湘雅二医院,湖南省长沙市410011 [3]江苏省苏北人民医院,江苏省扬州市225001

出　　处：《中国组织工程研究》2021年第1期141-145,共5页Chinese Journal of Tissue Engineering Research

基　　金：扬州市科技计划项目社会发展面上项目(YZ2017073),项目负责人:孙钰。

摘　　要：背景:层粘连蛋白可以促进干细胞增殖的特性已被予以广泛关注。目的:综述层粘连蛋白促进多种不同类型干细胞增殖的作用机制,为干细胞成软骨研究与应用提供可靠的理论依据。方法:检索中国期刊全文数据库(CNKI)、万方数据库、PubMed以及Web of Science等数据库2010年1月至2019年10月发表的相关文章,中文检索词为"层粘连蛋白""干细胞",英文检索词为"laminin""stem cell",筛选与层粘连蛋白作用机制、干细胞行为变化、软骨再生组织工程等内容紧密相关的文献,剔除重复、与主题相关性较差的文献,最后纳入57篇文献进行综述。结果与结论:①总结了层粘连蛋白的结构特点,分析整理了层粘连蛋白在软骨发育、退化过程中的时空变化,同时对比了天然软骨组织与组织工程软骨组织中层粘连蛋白表达的分布差异;②阐述了层粘连蛋白对胚胎干细胞、诱导多能干细胞、成体干细胞等的增殖调节作用;③提出了层粘连蛋白与干细胞共同用于软骨再生的热点方向,为未来软骨缺损与再生治疗提供理论依据。BACKGROUND:The characteristics of laminin that can promote the proliferation of stem cells have been widely concerned.OBJECTIVE:To review the interactions between laminin and many different stem cells,and provide reliable theoretical basis for chondrogenic research and application of stem cells.METHODS:Wanfang,CNKI,PubMed and Web of Science databases were searched for articles related to mechanism of laminin,changes in stem cell behaviors,and cartilage regeneration published from January 2010 to October 2019.The retrieval terms were“laminin”and“steam cells”in Chinese and English.Duplicated and poorly related articles were excluded,and finally 57 articles were included for review.RESULTS AND CONCLUSION:(1)The structural characteristics of laminin were summarized.The spatiotemporal changes of laminin during cartilage development and degradation were analyzed.At the same time,the distribution of laminin expression in natural cartilage tissue and tissue engineered cartilage tissue was compared.(2)The effects of laminin on the proliferation of various stem cells,including embryonic stem cells,induced pluripotent stem cells and adult stem cells,were described.(3)The possible hotspots on the combination of laminin and stem cells for cartilage regeneration were proposed,with the attempt of providing theoretical basis for cartilage repair and regeneration in the future.

关 键 词：干细胞 祖细胞 胚胎干细胞 成体干细胞 软骨 再生 层粘连蛋白 因子 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]