胰高血糖素样肽-1对2型糖尿病大鼠骨髓间充质干细胞成骨和成脂分化的影响  

Effect of glucagon⁃like peptide⁃1on osteogenesis and adipogenesis differentiation of bone marrow mesenchymal stem cells from rats in type 2 diabetes mellitus

作　　者：邓颖[1] 黎金凤[1] 陈志雄[1] 王晨秀[1] 胡雅琴[1] 黄水金 刘精东[1] 程宗佑[1] 霍亚南[1] DENG Ying;LI Jinfeng;CHEN Zhixiong;WANG Chenxiu;HU Yaqin;HUANG Shuijin;LIU Jingdong;CHENG Zongyou;HUO Yanan(Department of Endocrinology,Jiangxi Provincial People’s Hospital Affiliated to Nanchang University,Nanchang,Jiangxi 330000,China)

机构地区：[1]江西省人民医院、南昌大学附属人民医院内分泌科,江西南昌330000

出　　处：《安徽医药》2020年第9期1708-1711,I0002,共5页Anhui Medical and Pharmaceutical Journal

基　　金：江西省卫生计生委科技计划项目(20165024)。

摘　　要：目的探讨胰高血糖素样肽-1(GLP-1)对2型糖尿病(T2DM)大鼠骨髓间充质干细胞(BMSCs)成骨和成脂分化的作用。方法将20只SD大鼠按随机数字表法分为正常组及T2DM组,每组10只,高脂饲料喂养1个月联合链脲佐菌素(STZ)溶液注射制备T2DM模型大鼠,原代分离培养正常大鼠和T2DM大鼠BMSCs,并采用流式细胞术鉴定细胞。随后将正常大鼠BMSCs分为正常大鼠BMSCs(正常组),正常大鼠BMSCs+10 nmol/L GLP-1(正常组+10 nmol/L GLP-1),正常大鼠BMSCs+30 nmol/L GLP-1(正常组+30 nmol/L GLP-1)。T2DM模型大鼠BMSCs分为T2DM模型大鼠BMSCs(模型组),T2DM模型大鼠BMSCs+10nmol/L GLP-1(模型组+10 nmol/L GLP-1),T2DM模型大鼠BMSCs+30 nmol/L GLP-1(模型组+30 nmol/L GLP-1)。检测各组细胞的碱性磷酸酶(ALP)活性及成脂、成骨分化情况。结果分离获得的大鼠BMSCs细胞呈纺锤形或长梭形,核内可见1~2个核仁。流式细胞术结果表明正常组大鼠及模型组大鼠的BMSCs均一性较好,纯度高。与正常组(2.81±0.04)比较,正常组+10nmol GLP-1(4.92±0.07)及正常组+30 nmol GLP-1(6.76±0.02)的ALP活性升高,模型组(3.54±0.09)ALP活性降低;与模型组比较,模型组+10 nmol GLP-1(5.11±0.10)及模型组+30 nmol GLP-1(5.80±0.17)的ALP活性升高(F=23.56,P=0.000)。与正常组(0.46±0.05)比较,正常组+10 nmol GLP-1(0.53±0.05)及正常组+30 nmol GLP-1(0.64±0.02)的OD值升高,模型组(0.09±0.01)OD值降低;与模型组比较,模型组+10 nmol GLP-1(0.16±0.03)及模型组+30 nmol GLP-1(0.33±0.03)的OD值升高(F=18.39,P=0.001)。与正常组(0.29±0.05)比较,正常组+10 nmol GLP-1(0.16±0.05)及正常组+30 nmol GLP-1(0.09±0.02)的OD值降低,模型组(0.66±0.01)OD值升高;与模型组比较,模型组+10 nmol GLP-1(0.43±0.03)及模型组+30 nmol GLP-1(0.34±0.03)的OD值降低(F=29.41,P=0.000)。结论 GLP-1对T2DM大鼠BMSCs成骨分化具有促进作用,对成脂分化具有抑制作用。Objective To explore effect of glucagon⁃like peptide⁃1(GLP⁃1)on osteogenesis and adipogenesis differentiation of bone marrow mesenchymal stem cells(BMSCs)inrats with type 2 diabetes mellitus(T2DM).Methods Twenty SD rats were randomly divided into two groups:normal group and T2DM group according to the random number table method.T2DM rats was established by high⁃fat diet feeding for 1 month combined with streptozotocin(STZ)injection.BMSCs of normal rats and T2DM rats were isolat⁃ed and cultured in primary generation,and BMSCs were identified by flow cytometry.Normal rats BMSCs were divided into normal rats BMSCs(normal group),normal rats BMSCs+10 nmol/L GLP⁃1(normal group+10 nmol/L GLP⁃1),normal rats BMSCs+30 nmol/L GLP⁃1(normal group+30 nmol/L GLP⁃1).BMSCs of T2DM rats were divided into BMSCs of T2DM rats(model group),BMSCs+10 nmol/L GLP⁃1(model group+10 nmol/L GLP⁃1)and BMSCs+30 nmol/L GLP⁃1(model group+30 nmol/L GLP⁃1)in T2DM model rats.After glucagon⁃like peptide⁃1(GLP⁃1)was added to BMSCs of normal rats and model rats,Alkaline phospha⁃tase(ALP)activity,differentiation of BMSCs after induction in each group was detected.Results The isolated BMSCs cells were spindle⁃shaped or spindle⁃shaped with 1⁃2 nucleoli in the nucleus.The results of flow cytometry showed the BMSCs of normal group and model group had good homogeneity and high purity.Compared with normal group(2.81±0.04),the activity of ALP was increased in normal group+10 nmol/L GLP⁃1(4.92±0.07)and normal group+30 nmol/L GLP⁃1(6.76±0.02),the activity of ALP was decreased in model group(3.54±0.09).Compared with modelgroup,the activity of ALP was increased in normal group+10 nmol/L GLP⁃1(5.11±0.10)and normal group+30 nmol/L GLP⁃1(5.80±0.17)(F=23.56,P=0.000).Compared with normal group(0.46±0.05),OD value was increased in normal group+10 nmol/L GLP⁃1(0.53±0.05)and normal group+30 nmol/L GLP⁃1(0.64±0.02),OD value was decreased in model group(0.09±0.01).Compared with modelgroup,OD value was increased i

关 键 词：糖尿病 2型 间质干细胞 胰高血糖素样肽-1 成骨分化 成脂分化 大鼠 SPRAGUE-DAWLEY 

分 类 号：R587.1[医药卫生—内分泌]