反相高效液相色谱法测定人血清中拉莫三嗪的浓度  

Determination of lamotrigine in human serum by RP⁃HPLC

作　　者：王晓娟[1] 袁君[1] 黄庆 赵伍西[2] WANG Xiaojuan;YUAN Jun;HUANG Qing;ZHAO Wuxi(Department of Pharmacy,Huainan First People’s Hospital,Huainan,Anhui 232007,China;Department of Medicine,Huainan Union University,Huainan,Anhui 232007,China)

机构地区：[1]淮南市第一人民医院药学部,安徽淮南232007 [2]淮南联合大学医学系,安徽淮南232007

出　　处：《安徽医药》2020年第9期1732-1734,共3页Anhui Medical and Pharmaceutical Journal

基　　金：淮南市科技计划项目(2013A4307)。

摘　　要：目的建立人血清拉莫三嗪浓度的反相高效液相色谱方法。方法以艾司唑仑为内标,乙酸乙酯为萃取剂,C18柱分析柱,流动相甲醇∶磷酸二氢钠缓冲液(56∶44,p H 6.0),流速1.0 mL/min;检测波长210 nm,柱温30℃。结果拉莫三嗪在0.10~100.00 mg/L范围浓度内线性关系良好(r=0.999 6),定量浓度下限为0.10 mg/L,高、中、低质控样品相对回收率在80%~120%之间,提取回收率分别为90.95%、90.98%、95.10%,日内、日间相对标准偏差(RSD)均<5%。结论该方法准确,分析时间较短,线性范围大,可用于服用拉莫三嗪病人的血清浓度的临床常规监测。Objective To establish an Reversed⁃phase High Performance Liquid Chromatography(RP⁃HPLC)method for the con⁃centration of lamotrigine in human serum.Methods Estazolam was used as internal standard,ethyl acetate was used as extractant,and lamotrigine was analyzed via a C18 column and mobile phase methanol∶sodium hydrogen phosphate buffer(56∶44,pH 6.0)un⁃der the flow rate of 1.0mL/min,at the column temperature of 30℃.The detection occurred at wave length of 210nm.Results La⁃motrigine has a good linear relationship within the concentration range of 0.10⁃100.00 mg/L(r=0.9996)with a quantitative lower limit of 0.10 mg/L.The extraction recoveries rate of high,medium and low quality control samples was in the range of 80%⁃120%.The extraction recovery rates were 90.95%,90.98%and 95.10%respectively,,and the relative standard deviations(RSD)within and between days were<5%.The intraday and intraday precisions were less than 5%.Conclusion The method is accurate and sensitive,the analysis time is short,and the linear range is large.It can be used to detect the serum concentration of lamotrigine for its clinical applications.

关 键 词：拉莫三嗪/分析 色谱法 高压液相 血药浓度 

分 类 号：R969.1[医药卫生—药理学]