通痹方热敷联合针刺治疗对退变椎间盘细胞凋亡相关基因Caspase-3、Bcl-2 mRNA的影响  

Effects of Tongbi prescription hot compress combined with acupuncture on mRNA expressions of apoptosis-related genes,Caspase-3 and Bcl-2,in degenerative intervertebral discs

作　　者：徐银琴 史红美 王光义 Xu Yinqin;Shi Hongmei;Wang Guangyi(Guizhou Medical University,Guiyang 550004,Guizhou Province,China;Guizhou Provincial Staff Hospital,Guiyang 550025,Guizhou Province,China;Guizhou Medical University Affiliated Hospital,Guiyang 550004,Guizhou Province,China)

机构地区：[1]贵州医科大学,贵州省贵阳市550004 [2]贵州省职工医院,贵州省贵阳市550025 [3]贵州医科大学附属医院,贵州省贵阳市550004

出　　处：《中国组织工程研究》2021年第5期713-718,共6页Chinese Journal of Tissue Engineering Research

基　　金：贵州省中医药局中医药、民族医药科学技术研究课题(QZXY-2016-075),项目负责人:王光义;贵阳市科技计划项目[筑科合同(2019)9-1-4],项目负责人:王光义。

摘　　要：背景:研究表明,针刺及中药热敷治疗椎间盘退行性疾病具有良好的疗效,但2种方法联合应用对椎间盘细胞凋亡的影响尚不明确。目的:探讨通痹方热敷联合针刺治疗对颈椎病模型家兔椎间盘细胞凋亡相关基因Caspase-3、Bcl-2 mRNA的影响。方法:24只新西兰兔随机分为空白对照组、模型对照组、针刺治疗组、联合治疗组,各6只。除空白对照组外,其余3组制备颈椎病模型,造模成功后针刺治疗组采取针刺治疗30 min/(次·d)、联合治疗组给予通痹方热敷联合针刺治疗45 min/(次·d);于造模前、造模30 d、治疗结束后拍摄家兔颈椎X射线片并评分,治疗15次后留取各组兔C3-4、C4-5椎间盘。苏木精-伊红染色观察C3-4椎间盘组织形态学变化并评分,实时荧光定量PCR法检测C4-5椎间盘Caspase-3、Bcl-2 mRNA相对表达量。实验方案经贵州医科大学动物伦理委员会批准。结果与结论:①与模型对照组相比,针刺治疗组、联合治疗组颈椎病变减轻,X射线评分降低(P<0.05),联合治疗组评分与针刺治疗组比较无明显差别(P>0.05);②与模型对照组相比,针刺治疗组、联合治疗组椎间盘组织损伤减少,评分降低,且联合治疗组评分低于针刺治疗组(P<0.05);③针刺治疗组、联合治疗组较模型对照组Casepase-3 mRNA表达下降、Bcl-2 mRNA表达增加,联合治疗组较针刺治疗组Casepase-3 mRNA表达低、Bcl-2 mRNA表达高(P<0.05);④结果表明,通痹方热敷联合针刺治疗可延缓椎间盘退变,其机制可能与Bcl-2 mRNA表达增加,Casepase-3 mRNA表达减少有关。BACKGROUND:Acupuncture and hot compress of traditional Chinese medicine therapy have good curative effect on degenerative diseases of the intervertebral disc;however,the combination effect of these two methods on the apoptosis of intervertebral disc cells remains unclear.OBJECTIVE:To explore the effects of Tongbi prescription hot compress combined with acupuncture on the mRNA expression of apoptosis-related genes,Caspase-3 and Bcl-2,in intervertebral disc cells in a rabbit model of cervical spondylosis.METHODS:Twenty-four rabbits were randomly divided into normal group,model group,acupuncture group and combination treatment group,with six rabbits in each group.Except for the normal group,animal models of cervical spondylosis were established in the other three groups.After successful modeling,acupuncture treatment(30 minute once a day)and Tongbi Recipe hot compress combined with acupuncture therapy(30 minutes once a day)were done in the acupuncture group and combination treatment group,respectively.The cervical spine X-ray of each rabbit was taken and scored before modeling,at 30 days after molding as well as at the end of the treatment.The C3-4 and C4-5 intervertebral discs of each group were collected after treatment for 15 times.Hematoxylin-eosin staining was used to observe the morphological changes of C3-4 discs,and real-time quantitative PCR was used to detect the relative expression of Caspase-3 and Bcl-2 mRNA in C4-5 intervertebral discs.The study protocol was approved by the Animal Ethics Committee of Guizhou Medical University.RESULTS AND CONCLUSION:Compared with the model group,the cervical spine lesions in the acupuncture group and combination treatment group were significantly reduced,with a significant reduction in the X-ray scores(P<0.05).However,there was no significant difference between these treatment groups.The injury of intervertebral disc tissue and scores in the acupuncture group and combination treatment group were lower than those in the model group,and the score of the combination treatm

关 键 词：针刺 热敷 椎间盘 退变 细胞凋亡 蛋白 基因 实验 

分 类 号：R244.9[医药卫生—针灸推拿学] R274[医药卫生—中医临床基础]