针刀治疗胶原诱导性关节炎大鼠滑膜组织Bcl-2/Bax的表达  

Effect of acupotomy therapy on the expression of Bcl-2/Bax in synovial tissue of collagen-induced arthritis rats

作　　者：刘青 万碧江 Liu Qing;Wan Bijiang(College of Acupuncture and Moxibustion and Orthopedics,Hubei University of Traditional Chinese Medicine,Wuhan 430061,Hubei Province,China)

机构地区：[1]湖北中医药大学针灸骨伤学院,湖北省武汉市430061

出　　处：《中国组织工程研究》2021年第5期729-734,共6页Chinese Journal of Tissue Engineering Research

基　　金：湖北中医药大学研究生创新能力提升项目(1010040103)。

摘　　要：背景:临床研究表明,针刀治疗能够改善类风湿关节炎患者的症状,减轻关节肿胀,改善受损关节的活动功能,但尚无其具体作用机制的实验研究报道。目的:观察针刀治疗对胶原诱导性关节炎大鼠滑膜组织Bcl-2、Bax表达的影响。方法:将40只大鼠随机分为正常对照组10只,造模组30只。造模组大鼠采用二次免疫法制备胶原诱导性关节炎模型,随机选取20只造模成功大鼠随机分为模型组和针刀组。正常组和模型组不予处理;针刀组大鼠于左膝关节周围触及髌内外支持带、膝内外侧副韧带、髌底上缘中点及髌韧带的中点,每次选取上述2点予以针刀治疗,1次/周,共治疗3次。实验过程中监测大鼠一般情况,测量大鼠左后足趾厚度,进行关节炎指数评分;苏木精-伊红染色法观察关节滑膜病理形态;应用实时定量PCR法、免疫荧光技术分别检测滑膜组织Bcl-2、Bax的基因表达水平及免疫荧光强度。实验方案经湖北中医药大学动物实验伦理委员会批准(批准号为00127518)。结果与结论:①与正常组相比,模型组左后足趾厚度及关节炎指数评分明显增加(P<0.01,P<0.01);与模型组相比,针刀组左后足趾厚度及关节炎指数评分减小(P<0.05,P<0.05);②病理形态上,模型组较正常组滑膜层数增厚,大量炎症细胞浸润,针刀组较模型组滑膜层数减少,炎症细胞浸润减轻;③各组间滑膜组织Bax mRNA表达水平比较,差异无显著性意义(P>0.05);针刀组Bcl-2 mRNA、Bcl-2/Bax mRNA表达较模型组明显降低(P<0.01,P<0.01);④与模型组比较,针刀组Bcl-2/Bax荧光强度明显降低(P<0.01);⑤结果表明,针刀治疗类风湿关节炎的机制可能与下调滑膜组织中Bcl-2表达,降低Bcl-2/Bax比值有关。BACKGROUND:It has been shown that acupotomy therapy can improve the symptoms of rheumatoid arthritis,reduce the swelling of the joints,and improve the function of the damaged joints in clinical practice.However,the specific mechanism has not been reported in animal experiments.OBJECTIVE:To observe the effect of acupotomy therapy on the expression of Bcl-2 and Bax in synovial tissue of collagen-induced arthritis rats.METHODS:Forty rats were prepared for the experiment.Ten of them were randomly selected as normal controls,and the other 30 rats were used to manufacture collagen-induced arthritis models via secondary immunization.After the modeling,20 successful models were randomly selected for subsequent experiments and randomly divided into a model group and an acupotomy group,with 10 in each group.Normal group and model group remain untreated.The medial and lateral patellofemoral ligament,the tibial and fibular collateral ligament,the midpoint of the upper margin of the patella and the midpoint of the patellar ligament around the left knee joint were selected as points of acupotomy treatment,two of which were selected for each acupotomy session.Rats in the acupotomy group were treated once a week for 3 continuous weeks.The general condition of experimental rats was monitored daily,thickness of the left posterior toe was measured and arthritis index was evaluated.Hematoxylin-eosin staining was executed to observe the pathological morphology of the synovial tissue.Real-time quantitative PCR and immunofluorescence staining were implemented to measure gene expression and immunofluorescence intensity of Bcl-2 and Bax in synovial tissue,respectively.The study protocol was approved by the Animal Ethic Committee of Hubei University of Traditional Chinese Medicine with an approval No.00127518.RESULTS AND CONCLUSION:Compared with the normal group,the thickness of the left posterior toe and arthritis index score of the model group were significantly increased(P<0.01,P<0.01).Compared with the model group,the left hind toe th

关 键 词：针刀 关节炎 类风湿关节炎 滑膜 细胞凋亡 动物 鼠 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]