ABTS自由基显色分光光度法测定酵素中的黄酮  

ABTS^+·spectrophotometry for the determination of flavonoids in ferment

作　　者：王明瑞[1,2] 侯彦喜 叶超[1] WANG Mingrui;HOU Yanxi;YE Chao(College of Materials and Chemical Engineering,Kaifeng University,Kaifeng 475000,Hlenan,China;Kaifeng Engineering'Technology Research Center on Fermented Food,Kaifeng 475000,Henan,China)

机构地区：[1]开封大学材料与化学工程学院,河南开封475000 [2]开封市发酵食品工程技术研究中心,河南开封475000

出　　处：《化学研究》2020年第3期246-251,共6页Chemical Research

基　　金：开封市2020年科技发展计划项目(2002006).

摘　　要：2,2⁃联氮⁃二(3⁃乙基⁃苯并噻唑⁃6⁃磺酸)二铵盐(ABTS)在酸性条件下产生绿色稳定存在的ABTS+·,加入黄酮类物质后,ABTS+·在415 nm处的吸收峰降低,在337 nm处吸收峰显著增加.对测定条件进行优化后,建立了测定黄酮的工作曲线,对酵素样品中的黄酮进行测定.研究表明酵素中的黄酮类物质含量与337 nm处吸光度的增加量呈正比.当黄酮质量浓度(用芦丁表示)在0~2.00 mg/L范围内线性关系良好(R2=0.99998),最低检测限为0.04 mg/L.精密度、重复性试验结果的相对标准偏差(RSD)均低于2%,平均加标回收率为98.2%,RSD值为1.10%.研究表明该方法可靠、准确、重现性好,可快速测定酵素中的黄酮类含量.ABTS can produce green and stable ABTS+·in acidic condition.After adding flavonoids,the absorption peak of ABTS·+decreased at 415 nm and increased significantly at 337 nm.After the reaction conditions were optimized,the working curve was established and the ferment samples were determined.The results show that flavonoid content is directly proportional to the increase of absorbance at 337 nm.There is a good linear relationship between the mass concentration of flavonoid and the absorbance value in the range of 0-2.00 mg/L(correlation coefficient R2=0.99998),and the minimum detection limit is 0.04 mg/L.The relative standard deviation(RSD)of precision and repeatability test results is both less than 2%.The average recovery rate is 98.2%,with RSD of 1.10%,which indicates that the method is reliable,accurate and reproducible,and could be used for the rapid determination of flavonoid in fermented beverages.

关 键 词：发酵提取液 黄酮含量 ABTS自由基 等吸收点 

分 类 号：O657.32[理学—分析化学]