Plackett-Burman联用Box-Behnken响应面法优选镰形棘豆总多糖纯化工艺  

Purification Process Optimization of Total Polysaccharides from Oxytropis Falcat Bunge Based on Plackett-Burman Design Combined with Box-Behnken Response Surface Methodology

作　　者：蔡小辉 叶财发 刘晓玲[1] 陈锦珊[1] Cai Xiaohui;Ye Caifa;Liu Xiaoling;Chen Jinshan(Pharmacy Department,the 909th Hospital of the PLA,Affiliated Southeast Hospital of Xiamen University,Fujian Zhangzhou 363000,China)

机构地区：[1]中国人民解放军联勤保障部队第909医院/厦门大学附属东南医院药剂科,福建漳州363000

出　　处：《中国药师》2020年第8期1631-1636,共6页China Pharmacist

基　　金：原南京军区科技创新项目(编号:14MS085);漳州市自然科学基金项目(编号:ZZ2018J15);第九〇九医院青年苗圃项目(编号:17Y012、18Y010)。

摘　　要：目的:运用Plackett-Burman实验设计联合Box-Behnken响应面法,筛选大孔树脂法优化镰形棘豆中总多糖的纯化工艺。方法:通过单因素试验探讨各因素对镰形棘豆总多糖吸附率或洗脱率的影响。在此基础上,采用Plackett-Burman实验设计筛选影响镰形棘豆多糖回收率3个显著性因素,再采用Box-Behnken响应面法对这3个主要影响因素进行工艺优化,得到最佳纯化条件,并进行验证分析。结果:大孔树脂为ADS-7,径高比为1∶11,上样液浓度为0.2 mg·ml^-1,上样量为60 ml,上样速率为1.0 ml·min^-1,洗脱液为51%乙醇,洗脱量60 ml,洗脱速率为1.0 ml·min^-1,验证试验中镰形棘豆多糖平均回收率达87.03%,RSD为0.52%,与预测值的偏差为2.80%。通过该工艺镰形棘豆总多糖的纯度由19.2%提高到66.5%。结论:Plackett-Burman联用Box-Behnken响应面法优化的镰形棘豆多糖纯化工艺稳定可靠、简便易行,可为镰形棘豆总多糖的开发利用奠定基础。Objective: To optimize the purification technology of polysaccharides from Oxytropis falcat Bunge by Plackett-Burman design combined with Box-Behnken response surface methodology. Methods: The effects of various factors on the adsorption rate or desorption rate of total polysaccharides from Oxytropis falcat Bunge were investigated by single factor test. On the basis of single factor test,Plackett-Burman design was used to screen the factors affecting the recovery rate of total polysaccharides from Oxytropis falcat Bunge,and three significant factors were selected. And then,Box-Behnken response surface methodology was used to optimize the three main influencing factors to obtain the optimal purification conditions,and the verification analysis was carried out. Results: The optimal extraction purification conditions were as following: the macroporous resin was ADS-7,the ratio of diameter to height was 1 ∶ 11,the loading concentration was 0.2 mg·ml^-1,the loading volume was 60 ml,the loading rate was 1.0 ml·min^-1,the eluent was 51% ethanol,the eluent volume was 60 ml and the elution rate was 1.0 ml·min^-1. Under above conditions,the recovery rate of polysaccharides from Oxytropis falcat Bunge was up to 87.03%,and the deviation from the predicted value was 2.80%. Through the process optimization,the purity of total polysaccharides from Oxytropis falcat Bunge was increased from 19.2% to 66.5%. Conclusion: The purification technology of polysaccharides from Oxytropis falcat Bunge is stable,reliable and simple,which lays foundation for the development and utilization of polysaccharides.

关 键 词：镰形棘豆 总多糖 纯化工艺 PLACKETT-BURMAN设计 Box-Behnken响应面法 

分 类 号：R284.2[医药卫生—中药学]