含蒽硫杂杯[4]芳烃荧光探针的合成及其对Fe^3+的识别  

Synthesis of fluorescent probe containing bismuth thion [4] arene and its recognition of Fe^3+

作　　者：谢海芳 余昌金 黄亚励 徐红 张奇龙 孙晓红 XIE Haifang;YU Changjin;HUANG Yali;XU Hong;ZHANG Qilong;SUN Xiaohong(School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 550025;School of Biology and Engineering,Guizhou Medical University,Guiyang 550025;School of Basic Medical Sciences,Guizhou Medical University,Guiyang 550025;School of Food Safety,Guizhou Medical University,Guiyang 550025)

机构地区：[1]贵州医科大学公共卫生学院,环境污染与疾病监控教育部重点实验室,贵阳550025 [2]贵州医科大学生物与工程学院,贵阳550025 [3]贵州医科大学基础医学院,贵阳550025 [4]贵州医科大学食品安全学院,贵阳550025

出　　处：《分析试验室》2020年第8期926-930,共5页Chinese Journal of Analysis Laboratory

基　　金：贵州省区域内一流学科建设项目-公共卫生与预防医学(黔教科研发[2017]85号)项目资助。

摘　　要：合成含蒽硫杂杯[4]芳烃荧光探针A,通过1H NMR和13C NMR对其结构进行了表征,考察了探针的光谱性能及其对常见离子的选择性识别,并进行了实际水样的加标回收检测。结果表明,在四氢呋喃/Tris-HCl缓冲溶液(4:1,V:V,pH 7.0)中,该探针可选择性识别Fe^3+,且受常见金属阳离子和阴离子的干扰较小;Fe^3+的浓度在0.05~0.50 mmol/L范围内,荧光探针A的荧光强度与Fe^3+的浓度呈现较好的线性关系,相关系数(R^2)为0.99696,检出限(LOD)为0.75μmol/L;荧光探针A的荧光强度受pH的影响很小,在较宽的pH范围内,仍可有效地检测Fe^3+。该荧光探针A用于实际样品中Fe^3+检测,回收率和相对标准偏差(RSDs)均达到要求,有望用于水环境和生命体系中Fe3+的选择性检测。The ruthenium-containing cup[4]arene fluoresceut probe A was synthesized and its structure was characterized by 1H NMR and 13C NMR.The spectral properties of the probe and its selective recognition of common ions were investigated.And the actual water sample and the spiked water samples were detected.The results showed that in THF/tris-hcl buffer solution with a volume ratio of 4:1(pH 7.0),the probe could selectively recognize Fe^3+ with high sensitivity,and was less interfered by common metal cation and anion.The concentration of Fe^3+ was in the range of 0.05-0.50 mol/L,the fluorescence intensity of fluorescent probe A showed a good linear relationship with the concentration of Fe^3+ with correlation coefficient R^2=0.99696.The detection limit was 0.75μmol/L.In a wide p H range,the fluorescence intensity of the fluorescent probe A was little affected by pH,and Fe^3+ could still be effectively detected.The probe A can be used for Fe^3+ detection in actual samples,and the recovery and RSD are both satisfied.It is expected to be used for selective and accurate detection of Fe^3+ in water environment and life system.

关 键 词：铁离子 荧光探针 硫杂杯[4]芳烃 金属离子识别 

分 类 号：O657[理学—分析化学]