宽pH响应范围的比率荧光纳米探针的构建  

Construction of ratiometric fluorescent nanoprobes with a wide range of pH response

作　　者：周楷[1] 刘玮[1] 郑国灿[1] 饶历 ZHOU Kai;LIU Wei;ZHENG Guocan;RAO Li(Analytical and Testing Center,Chongqing University,Chongqing 401331;School of Pharmaceutical Science,Chongqing University,Chongqing 401331)

机构地区：[1]重庆大学分析测试中心,重庆401331 [2]重庆大学药学院,重庆401331

出　　处：《分析试验室》2020年第8期931-935,共5页Chinese Journal of Analysis Laboratory

基　　金：重庆市教委科学技术研究计划项目(KJQN201800113)资助。

摘　　要：基于化学剥离法制备MoS2量子点,以荧光素和罗丹明B为pH荧光响应基团,经巯基共价修饰在MoS2量子点表面后构建了宽pH检测范围的比率荧光纳米探针dl-MoS2。对其光谱学性质和pH响应荧光性质进行了研究。结果表明,dl-MoS2具有pH选择性识别作用,且对pH响应范围可以通过荧光配体和罗丹明配体的比例进行调节,当荧光素配体与罗丹明配体的摩尔比为1:10时,制备得到的dl-MoS2探针具有最宽的pH响应范围(pH 4~9),该方法能有效的扩宽荧光探针的pH检测范围。进一步研究表明,dl-MoS2探针对pH的检测可用作生命体内pH成像检测的荧光探针。A double-labeled ratiometric fluorescent probe(dl-MoS2)with pH selective recognition was constructed based on MoS2 quantum dots which was preparatied by chemical exfoliation with fluorescein and rhodamine ligands.The spectroscopic properties of dl-MoS2 were investigated in detail.The experimental results indicated that the dl-MoS2 possesses the widest range of pH detection(pH 4-9)when the ratio of fluorescein ligand to rhodamine ligand was 1:10.This method could broaden the pH detection range of fluorescent probes effectively.Further research showed that the dl-MoS2 might be developed for pH imaging in vitro and in vivo.

关 键 词：硫化钼量子点 比率荧光 纳米探针 PH检测 

分 类 号：O661.1[理学—分析化学]