hsa-miR-4483促进人乳腺癌细胞系的增殖和迁移  

hsa-miR-4483 promotes the proliferation and migration of breast cancer cell lines

作　　者：阳德全 李常有 崔军威[2] 韦伟[2] 王少平 YANG De-quan;LI Chang-you;CUI Jun-wei;WEI Wei;WANG Shao-ping(Department of Hepatobiliary Surgery, the Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen 518000;Department of Thyroid and Breast Surgery, Peking University Shenzhen Hospital,Shenzhen 518000, China)

机构地区：[1]中山大学附属第八医院肝胆外科,广东深圳518000 [2]北京大学深圳医院甲乳外科,广东深圳518000

出　　处：《基础医学与临床》2020年第9期1230-1236,共7页Basic and Clinical Medicine

基　　金：深圳市科技项目(JCYJ20170307111713483)。

摘　　要：目的探讨hsa-miR-4483在乳腺癌细胞系中表达及作用。方法利用TargetScan找出hsa-miR-4483可作用c-Myc基因;利用RT-qPCR对hsa-miR-4483的表达量进行验证。转染MCF-7和MDA-MB-231,并设置NC阴性对照,Western blot检测hsa-miR-4483作用c-myc基因的表达量,利用MTT法和Transwell小室法验证乳腺癌细胞的增殖及迁移的能力。结果hsa-miR-4483在MCF-7和MDA-MB-231中显著低表达(P<0.001,P<0.01);MCF-7和MDA-MB-231的c-myc蛋白表达量上调(P<0.05);hsa-miR-4483促进了MCF-7和MDA-MB-231细胞增殖和迁移(P<0.001)。结论hsa-miR-4483可促进了乳腺癌细胞系的增殖以及迁移,其在乳腺癌的发展及其预后中起到调控的作用。Objective To explore the expression and function of hsa-miR-4483 in breast cancer cells.MethodsUsing TargetScan to find out how can-miR-4483 act on the c-Myc gene,and RT-qPCR verified the expression of hsa-miR-4483.Transfected MCF-7 and MDA-MB-231 and set up NC-negative controls,Western blot was used to detect the expression of the hsa-miR-4483 gene,MTT assay and Transwell chamber experiment were applied to verify the proliferation and migration of breast cancer cells.Results The expression of hsa-miR-4483 was significantly lower(P<0.001,P<0.01)in MCF-7 and MDA-MB-231(P<0.001,P<0.01)The up-regulation of c-myc in MCF-7 and MDA-MB-231 promoted cell proliferation and migration(P<0.001).Conclusions hsa-miR-4483 can promote the proliferation and migration of breast cancer cell lines,which plays a regulatory role in the development of breast cancer and its prognosis.

关 键 词：MICRORNA 乳腺癌 增殖 迁移 增殖曲线 

分 类 号：R655.8[医药卫生—外科学]