Cajal间质细胞数量在反复膀胱炎大鼠膀胱中增加  

Number increase of Cajal interstitial cells in bladder of rats with recurrent cystitis

作　　者：高峰[1] 张士更[2] 张楠[2] 文甲明[2] 虞旗旗[1] 黄亚胜[1] GAO Feng;ZHANG Shi-geng;ZHANG Nan;WEN Jia-ming;YU Qi-qi;HUANG Ya-sheng(Department of Urology,Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007;Department of Urology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China)

机构地区：[1]杭州市中医院泌尿外科,浙江杭州310007 [2]浙江大学医学院附属第二医院泌尿外科,浙江杭州310009

出　　处：《基础医学与临床》2020年第9期1237-1241,共5页Basic and Clinical Medicine

基　　金：杭州市科技发展计划项目(20191203B138)。

摘　　要：目的研究Cajal间质细胞(ICCs)在大鼠反复膀胱炎相关膀胱过度活动症(OAB)膀胱中的数量变化。方法将大鼠分为对照组和实验组[大肠埃希菌(E.coli)1×10^8~1.2×10^8 CFU/mL,100μL膀胱灌注],通过尿检和尿流动力学测定及HE染色膀胱组织明确OAB建模成功;用免疫荧光法观察膀胱组织c-kit阳性的ICCs,Western blot检测干细胞因子(SCF)及c-kit蛋白表达水平。结果实验组大鼠较对照组ICCs网格状分布紊乱,数量明显增加(P<0.05),干细胞因子(SCF)及c-kit蛋白表达明显升高(P<0.05)。结论ICCs在反复膀胱炎膀胱中明显升高。Objective To explore the changes of interstitial cells of Cajal(ICCs)in the over active bladder(OAB)rats caused by recurrent cystitis.Methods Rats were divided into control group and experimental group(E.coli 1×10^8~1.2×10^8 CFU/mL,100μL bladder perfusion),The OAB model was confirmed by urine test,urine flow dynamics and HE staining of bladder tissue.The immunofluorescence staining method was performed to detect c-kit positive ICCs in bladder tissue.Western blot was used to determine the expression of SCF/c-kit protein.ResultsCompared with the control group,the quantity of ICCs in the experimental group increased significantly(P<0.05),and the protein expression of SCF and c-kit increased significantly(P<0.05).Conclusions ICCs increase significantly in the bladder with recurrent cystitis.

关 键 词：膀胱过度活动症 卡哈尔(Cajal)间质细胞 干细胞因子 c-kit受体酪氨酸激酶 

分 类 号：Q491.1[生物学—生理学]