水稻早衰突变体w14的鉴定和病程相关基因表达分析  

Resistance to Early Senescence and Rice Blast/Blight:A Mutant Variety and Expressions of Responsible Genes

作　　者：朱永生 徐靖[2] 蒋家焕[3] 郑燕梅[1,3] 陈丽萍 蔡秋华[3] 王爱荣[1] 张建福[1,3] ZHU Yongsheng;XU Jing;JIANG Jiahuang;ZHENG Yanmei;CHEN Liping;CAI Qiu-hua;WANG Airong;ZHANG Jianfu(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Institute of Cereal Crops,Hainan Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding of Hainan Province,Haikou,Hainan 571100,China;Rice Research Institute,Fujian Academy of Agricultural Sciences/Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China,Ministry of Agriculture,P.R.China/Fuzhou branch,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding,Fuzhou,Fujian 350003,China)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]海南省农业科学院粮食作物研究所/海南省农作物遗传育种重点实验室,海南海口571100 [3]福建省农业科学院水稻研究所/农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州(国家)水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室,福建福州350003

出　　处：《福建农业学报》2020年第6期626-632,共7页Fujian Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0101801);海南省农作物遗传育种重点实验室开放课题项目(YCY201802);福建省自然科学基金项目(2018J01039)。

摘　　要：【目的】叶片早衰影响水稻的产量和品质,早衰突变体是研究水稻衰老机制的良好载体,鉴定、分析早衰突变体的表型特征,有助于了解突变体的遗传规律,为相关基因的克隆和功能研究奠定基础。【方法】人工接种鉴定了早衰突变体w14的稻瘟病和水稻白叶枯病抗性,同时通过对离体叶片的黑暗诱导和叶绿素含量测定,鉴定了突变体w14的早衰表型;再分别对早衰突变体w14和野生型粳稻品种云引(简称‘YY’)中水稻早衰相关基因和病程相关基因的表达进行分析,研究早衰突变体w14的早衰类型及与野生型在防御系统上的差异。【结果】黑暗诱导处理24 h后,突变体w14的叶绿素含量显著低于野生型YY(P<0.05)。黑暗诱导处理48 h以上,突变体w14的叶绿素含量极显著低于野生型YY(P<0.01)。突变体w14中衰老相关基因SGR、Osh36、Osh69、PAO、NYC3和RCCR1的表达均显著高于野生型YY。分别接种水稻白叶枯和稻瘟病后,突变体w14均表现为比野生型YY更感病,且病程相关基因PR1a、PR4、Cth1、PR1b、PBZ1、PR3等的表达在突变体中显著上调。【结论】突变体w14具有典型的衰老和感稻瘟病、水稻白叶枯病的表型,与野生型相比,在突变体中与病程相关基因的表达发生了显著改变,即突变体防御系统的改变导致其出现感病的表型。【Objective】A mutant rice showing signs of early senescence on leaves and susceptibility to rice blast and blight was used for phenotypic and genetic analyses to study the genes associated with the aging mechanism and vulnerability to the diseases of rice plants.【Method】Resistance to blast and bacterial blight of the mutant rice w14 with symptoms of early senescence was tested by artificial inoculation.Phenotype classification of the mutant was determined by chlorophyll content measured in vitro under induced darkness.Expressions of the genes related to senescence and pathogenesis in the mutant and wild-type japonica rice variety,Yunyin(YY),were compared.【Result】After inducing darkness for 24h,the chlorophyll content in w14 was 5% lower than that in YY,and the difference reached a 1% statistical significance level in 48 h.The expressions of senescence-related genes SGR,Osh36,Osh69,PAO,NYC3,and RCCR1 in w14 were significantly higher than those in the wild-type counterpart.The mutant rice was more susceptible to the artificial inoculations of rice blast and bacterial blight,and the pathogenesis-related genes PR1a,PR4,Cth1,PR1b,PBZ1,and PR3 significantly up-regulated in w14 than in YY.【Conclusion】The mutant rice w14 showed the typical senescence phenotypes with a higher susceptibility to rice blast and bacterial blight than the wild-type YY.The expressions of pathogenesis-related genes in the mutants were significantly altered that substantially affected its defense system leading to the early senescence and disease susceptibility as observed in the study.

关 键 词：水稻 突变体 早衰 叶绿素 抗病性 基因表达 

分 类 号：S511[农业科学—作物学]