杨梅苷毒性早期评价研究  

Early stage toxicity evaluation of myricitrin

作　　者：丁涛[1] 王鑫[1] 刘博[1] 温富春[1] 纪凤兰[1] 孙桂波[2] 潘瑞乐[2] 徐惠波[1] DING Tao;WANG Xin;LIU Bo;WEN Fu-chun;JI Feng-lan;SUN Gui-bo;PAN Rui-le;XU Hui-bo(Jilin Academy of Traditional Chinese Medicine,Changchun Jilin 130012,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China)

机构地区：[1]吉林省中医药科学院药效毒理评价中心,吉林长春130012 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《毒理学杂志》2020年第3期237-240,245,共5页Journal of Toxicology

基　　金：国家重大新药创制(2012ZX09103201-004)。

摘　　要：目的通过毒性早期筛选方法,初步明确化合物杨梅苷是否存在毒性,寻找其毒性靶器官和安全应用范围。方法采用急性毒性试验、为期1个月的重复给药毒性试验、遗传毒性(Ames试验和骨髓微核试验)等方法,考察杨梅苷的毒性。结果急性毒性试验结果表明,受杨梅苷溶解性的限制,未能测出半数致死量(LD50)值,杨梅苷1 d内最大给药量为7.2 g/kg·bw。为期1个月的反复给药毒性结果可见杨梅苷对动物行为状态、饮食饮水量及动物的生长均没有明显的影响;血液学结果可见雄性动物各剂量给药组HGB含量明显降低,高剂量给药组RBC亦降低;血液生化学结果显示,杨梅苷给药后引起动物的血糖波动,结合脏器指数的变化、病理组织学结果分析,虽个别指标差异有统计学意义,但未见明显的生物学意义。遗传毒性结果表明杨梅苷没有明显的致突变作用。结论本实验条件下,杨梅苷在0.225~1.2 g/kg·bw的剂量范围内,对机体未见明显的连续给药毒性反应或遗传毒性。Objective By the early stage screening method of toxicity, to find the toxic target organ and the safe range and preliminary definit whether myricitrin had the toxicity. Methods The toxic of myricitrin was inspected through acute toxicity test, a month-term repeated-dose toxicity test,Ames test and bone marrow cell micronucleus test. Results The result of the acute toxicity showed LD50 was not detected because of limiting by the solubility,but the maximum intragastric dose in a day was 7.2 g/kg·bw. The result of repeated-dose toxicity showed the behavior,dietary,water intake and growth of the animals had not significantly effected. Hematological result showed the contents of HGB in all male groups were significantly decreased, RBC of the male high dose group was also decreased. The blood biochemical index showed blood glucose fluctuation. These changes had no obvious physiological significance through analysing the organ indexs and pathological result, although some indicators had statistical sigificance. The genotoxic result showed myricitrin had no obvious mutagenicity. Conclusion Under current test condition, myricitrin had no obvious repeated-dose toxicity or genotoxicity in dosage range of 0.225~1.2 g/kg·bw.

关 键 词：杨梅苷 急性毒性 长期毒性 遗传毒性 

分 类 号：R996.2[医药卫生—毒理学]