蜂王浆对顺铂诱导HEK-293细胞损伤的保护作用及其机制研究  

Protective effect of royal jelly on cisplatin-induced damage to HEK-293 cells and its mechanism

作　　者：李佳 喻凯[1] 何雨轩 冯彤彤 王红 陆群[1] LI Jia;YU Kai;HE Yu-xuan;FENG Tong-tong;WANG Hong;LU Qun(College of Life Science and Engineering,Southwest Jiaotong University,Chengdu Sichuan 610031,China)

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《毒理学杂志》2020年第3期241-245,共5页Journal of Toxicology

基　　金：国家发改委中国西南药用昆虫资源开发协同创新研究基金资助(VQ99QT1113Y17001)。

摘　　要：目的探讨蜂王浆对顺铂诱导人胚肾细胞损伤的保护作用及其可能的机制。方法将细胞分为损伤组(100μg/ml CDDP)、蜂王浆(RJ)不同剂量组(CDDP+25、50、100μg/ml RJ)和正常对照组(等量溶剂)。各组经处理后,通过CCK-8法检测细胞增殖活力,比色法测定培养基乳酸脱氢酶(LDH)活性反映细胞损伤情况;Hoechst33342/PI双染法分析细胞凋亡情况;测定细胞活性氧(ROS)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果与对照组比较,损伤组培养液中LDH活性增加,细胞损伤增加、增殖活力降低、凋亡增加,差异具有统计学意义(P<0.05);细胞内SOD、CAT和GSH-Px活性降低,ROS含量增加,差异具有统计学意义(P<0.05)。25~100μg/ml浓度范围内RJ能明显改善这些指标,差异有统计学意义(P<0.05,P<0.01),且改善作用和浓度呈正相关(P<0.05)。结论 100μg/ml CDDP可通过氧化损伤诱导的HEK-293细胞凋亡,活力降低。25~100μg/ml浓度范围内RJ能增加胞内SOD、CAT和GSH-Px 3种抗氧化酶活性,降低ROS含量,减轻这种损伤。Objective To explore the protective effect and its mechanism of royal jelly(RJ) on cisplatin(CDDP)-induced damage of human embryonic kidney cells(HEK-293). Methods The cells were divided into injury group(100 μg/ml CDDP), RJ groups(CDDP+25, 50, 100 μg/ml RJ), and normal control group(equivalent solvent). The cells were treated according to the groups.The proliferation was tested with CCK-8.The activity of lactate dehydrogenase(LDH) in the medium was measured. Hoechst33342/PI double staining method was used to analyze the apoptosis.The reactive oxygen species(ROS), superoxide dismutase(SOD), catalase(CAT) and glutathione peroxidase(GSH-Px) in the cells were measured. Results Compared with the control group, the LDH activity in the culture medium of the injury group increased, the cell damage increased, the proliferation activity decreased, and the apoptosis increased, the difference was statistically significant(P<0.05);the intracellular SOD, CAT and GSH-Px activity decreased, The content of ROS increased and the difference was statistically significant(P<0.05). RJ in the concentration range of 25~100 μg/ml can significantly improve these indicators, the difference is statistically significant(P<0.05,P<0.01), and the improvement and concentration are positively correlated(P<0.05). Conclusion CDDP(100 μg/ml) can induce apoptosis of HEK-293 cells, decrease their viability due to oxidative damage. RJ(25~100 μg/ml) can increase the activity of antioxidant enzymes, SOD, CAT and GSH-Px, reduce ROS content, and alleviate the damage.

关 键 词：蜂王浆 HEK-293细胞 顺铂 细胞凋亡 抗氧化 

分 类 号：R965[医药卫生—药理学] R99[医药卫生—药学]