宁夏甜瓜白粉病室内苗期抗病性鉴定方法及抗性种质资源筛选  

Screenings of the identification method at seedling stage in indoor and the resistant germplasm resources of muskmelon to powdery mildew in Ningxia

作　　者：王禄星 王晓敏[1,2,3] 宋建宇 张雪艳 田梅[4] 于蓉 WANG Luxing;WANG Xiaomin;SONG Jianyu;ZHANG Xueyan;TIAN Mei;YU Rong(School of Agriculture,Ningxia University,Yinchuan 750021,Ningxia,China;Ningxia Modern Facility Horticulture Engineering Technology Research Center,Yinchuan 750021,Ningxia,China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan 750021,Ningxia,China;Institute of Germplasm Resource,Ningxia Academy of Agriculture and Forestry Science,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏现代设施园艺工程技术研究中心,银川750021 [3]宁夏优势特色作物现代分子育种重点实验室,银川750021 [4]宁夏农林科学院种质资源研究所,银川750002

出　　处：《中国瓜菜》2020年第8期11-15,共5页China Cucurbits And Vegetables

基　　金：宁夏回族自治区自然科学基金项目(NZ15021)。

摘　　要：为了给宁夏甜瓜白粉病防治与白粉病抗病育种的有效开展提供依据,采用不同接种苗龄、不同接种浓度、不同接种方法、接种后不同保湿时间四因素三水平(L9(3)^4)正交试验设计筛选甜瓜白粉病室内苗期抗性鉴定方法,并在此基础上在宁夏地区对23份甜瓜种质资源进行白粉病抗病性评价。结果表明,苗龄为4叶1心,孢子悬浮液浓度10^5个·mL^-1,摩擦法接种,接种后保湿36 h的处理组合为最佳的室内苗期抗病性鉴定方法;综合室内和田间鉴定结果,23份甜瓜种质资源中对白粉病免疫的材料有‘抗病F-3800’和‘1489’,发病率和病情指数均为0;高抗材料有‘新密45号’‘1464’‘绿洲红’‘Sweet’和‘美玉25号’,发病率低且病情指数均在10以下。To provide resistance germplasm and identification methods for melon breeding with resistance to powdery mildew,the resistance identification methods of muskmelon to powdery mildew at seedling stage in indoor were screened by using different inoculation methods,seedling stages,spore suspension concentrations and moisture periods which were designed by four factors and three levels(L9(3)^4)orthogonal experimental.On this basis,23 muskmelon germplasm resources were evaluated for powdery mildew resistance in Ningxia.The results showed that the optimal indoor resistance identification method of muskmelon to powdery mildew at seedling stage was the combination of 4-leaf stage,10^5·mL^-1 of the spore suspension concentration,friction inoculation and 36 h moisture.Combined results on resistance identification both in indoor and fields‘Kangbing F-3800’and‘1489’were immune,‘Xinmi 45’‘1464’‘Oasis Red’‘Sweet’and‘Meiyu 25’were high resistant.

关 键 词：甜瓜 白粉病 鉴定方法 正交试验 抗性鉴定 

分 类 号：S652[农业科学—果树学]