气相串接质谱联用法确证人尿中44种外源性蛋白同化类激素  

Confirmation of Forty-four Exdogenous Anabolic Androgenic Steroids in Human Urine Using Gas Chromatography-Mass Spectrometry

作　　者：景晶[1] 王杉[1] 刘欣[1] 杨声[1] 邓静[1] 张玉梅[1] 杨志勇[1] 张力思[1] 张亦农[1] Jing Jing;Wang Shan;Liu Xin;Yang Sheng;Deng Jing;Zhang Yumei;Yang Zhiyong;Zang Lisi;Zhang Yinong(National Anti-doping Laboratory,China Anti-doping Agency,Beijing 100029,China)

机构地区：[1]国家体育总局反兴奋剂中心,北京100029

出　　处：《中国运动医学杂志》2020年第6期476-481,共6页Chinese Journal of Sports Medicine

摘　　要：目的:采用气相色谱串接质谱联用技术建立人尿中包括脱氢氯甲睾酮、美雄酮、司坦唑醇等在内的44种外源性蛋白同化类激素的确证方法。方法:根据世界反兴奋剂机构(以下简称WADA)公布的2015年版技术文件Minimum criteria for chromatographic-mass spectrometric confirmation of the identity of analysis for doping control purposes(简称TD2015IDCR)的要求作为判定标准,方法验证重点考察选择性和特异性、提取回收率和稳定性等方面。结果:经验证,该方法特异性、选择性良好,符合TD2015IDCR关于确证方法的要求。结论:经过验证,该方法可检测人尿中44种外源性蛋白同化类激素,方法稳定可靠,科学有效。Objective To confirm forty-four exdogenous anabolic androgenic steroids including the de-hydrochloromethyltestosterone,metandienone,stanozolol in the human urine using the gas chromatogra-phy-mass spectrometry(GC-MS). Method According to the minimum criteria for chromatographic-massspectrometric confirmation of the identity of analysis for doping control purposes(TD2015 IDCR) pub-lished by World Anti-Doping Agency(short for WADA),the specificity and selectivity,recovery and sta-bility were investigated. Result The method had good selectivity and specificity,meeting the require-ments for confirmation recorded in the TD2015 IDCR.Conclusion The method on study is verified to beeffective and suitable for the detection of forty-four exogenous anabolic androgenic steroids in the hu-man urine.

关 键 词：兴奋剂检测 气相串接质谱 蛋白同化类激素 

分 类 号：R446.12[医药卫生—诊断学]