川麦42和川农16抗穗发芽QTL定位及聚合效应分析  

Identification and Pyramiding of QTLs for Traits Associated with Pre-Harvest Sprouting Resistance in Two Wheat Cultivars Chuanmai 42 and Chuannong 16

作　　者：王琴[1,2] 刘泽厚 万洪深 魏会廷[2,3] 龙海 李涛 邓光兵[4] 李俊[1,2] 杨武云 WANG Qin;LIU ZeHou;WAN HongShen;WEI HuiTing;LONG Hai;LI Tao;DENG GuangBing;LI Jun;YANG WuYun(Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066;Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China,Ministry of Agriculture,Chengdu 610066;Plant Protection Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041)

机构地区：[1]四川省农业科学院作物研究所,成都610066 [2]农业部西南地区小麦生物学与遗传育种重点实验室,成都610066 [3]四川省农业科学院植物保护研究所,成都610066 [4]中国科学院成都生物研究所,成都610041

出　　处：《中国农业科学》2020年第17期3421-3431,共11页Scientia Agricultura Sinica

基　　金：国家小麦产业技术体系(CARS-03);四川省科技计划(2016NYZ0049);四川省财政创新能力提升工程(2016ZYPZ-016)。

摘　　要：【目的】小麦穗发芽严重影响小麦产量和品质,是全球小麦生产面临的重大问题之一。通过鉴定挖掘抗穗发芽QTL,聚合穗发芽抗性位点,选育抗穗发芽小麦品种,为四川小麦穗发芽抗性改良提供技术和材料支撑。【方法】以川麦42/川农16重组自交系(RIL,F8)为材料,于2016-2018年分别在2个环境下对RIL群体进行籽粒发芽指数(GI,2016和2018)、籽粒发芽率(GR,2016和2018)和整穗发芽率(SGR,2017和2018)3个穗发芽指标测定。利用90K SNP芯片构建的遗传图谱检测全基因组穗发芽相关QTL,并分析抗性QTL聚合效应。【结果】双亲间GI、GR和SGR指标值差异显著,亲本川农16穗发芽抗性明显优于亲本川麦42。共检测到11个与穗发芽抗性有关的QTL,主要分布在2B、2D、3A、3D、4A、5A、5B和6B染色体上。5B染色体上检测到的单个环境表达的整穗发芽QTL解释的表型变异率最大,达到29%;在2D和3A染色体上检测到的整穗发芽主效QTL,以及5A染色体上检测到的与种子休眠相关的籽粒发芽主效QTL,在2个环境下均能表达,其抗穗发芽等位变异均来源于川农16。基因型分析发现,RIL群体中不同株系聚合抗性QTL的数量变幅为1-9个,表现为抗穗发芽的株系均携带4—9个与穗发芽相关的抗性QTL。重组自交系群体中6个株系GI、GR和SGR值均在15%以下,表现出高抗穗发芽特性;这6个优异株系聚合了多个与穗发芽相关的抗性QTL,且均聚合了川麦42在4A染色体上的微效QTL(QGi.saas-4A和QGr.saas-4A),以及川农16在2D和5B染色体上的主效QTL(QSgr.saas-2D和QSgr.saas-5B);编号为104和125的优异株系已通过审定,定名为川麦104和川麦64。其中,川麦104于2012年同时通过国家和四川省审定,其抗穗发芽能力强,产量、品质、抗病等优良性状突出,聚合了7个正向穗发芽QTL,包括2B、2D和5B染色体上来源于川农16的4个抗性QTL(QGi.saas-2B、QGr.saas-2B、QSgr.saas-2D和QSgr.saas-5B),以及4A和6B染�【Objective】Pre-harvest sprouting(PHS)is one of the serious problems for wheat production,which significantly reduces grain yield and end-use quality,especially in rainy or high humidity regions.The objective of this study is to identify and aggregate quantitative trait loci(QTLs)for traits associated with PHS resistance,which will provide a theoretical basis for improving PHS resistance in Sichuan wheat cultivars.【Method】A recombinant inbred line(RIL)population derived from Chuanmai 42 and Chuannong 16 was used to detect QTL and assess the new germplasm resources for PHS resistance.127 lines in RIL population were analyzed by phenotypic and genetic identification for PHS-related traits.Seed germination index(GI),seed germination rate(GR)and seed germination rate of in each spike(SGR)in two different environments were used to evaluate PHS resistance.All QTLs for PHS resistance were mapped by an available high-density single nucleotide polymorphism(SNP,90K).The pyramiding of the resistant QTL was also analyzed according to the genotype of every line in RILs.【Result】There were significant difference in GI,GR and SGR between two parents.PHS resistance of Chuannong 16 was superior than that of Chuanmai 42.A total of 11 QTLs for PHS were detected on chromosomes 2B,2D,3A,3D,4A,5A,5B and 6B.QSgr.saas-5B was significantly associated with PHS resistance in single environment and explained 29.03%phenotypic variation.QSgr.saas-2D,QSgr.saas-3A,QGi.saas-5A and QGr.saas-5A could express stably in two environments,and the alleles of enhancing PHS resistance were from Chuannong 16.The results of genotype analysis showed that the number of resistant QTL in different lines ranged from one to nine.Six excellent lines in RILs with high resistance carried seven or eight additive QTLs for PHS resistance.These additive QTLs included the minor QTLs on chromosome 4A from Chuanmai 42 and the major QTLs on chromosomes 2D and 5B from Chuannong 16.No.104 and No.125 in RIL population were released in China or Sichuan province becau

关 键 词：小麦 穗发芽 QTL定位 QTL聚合 聚合效应 

分 类 号：S512.1[农业科学—作物学]