腕踝针对坐骨神经痛大鼠脊髓背角谷氨酸及N-甲基-D-天冬氨酸受体磷酸化蛋白表达的影响  

Effect of wrist-ankle acupuncture on the expression of glutamate and NMDA receptor of the spinal dorsal horn in rats with neuropathic pain

作　　者：柳伟婷 江孟鸿 王志福[2] 郑美凤[1] 陈小梅 张亮平 梁胜祥 俞向梅[2] LIU Wei-ting;JIANG Meng-hong;WANG Zhi-fu;ZHENG Mei-feng;CHEN Xiao-mei;ZHANG Liang-ping;LIANG Sheng-xiang;YU Xiang-mei(School of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;School of Integrated Traditional Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;School of Rehabilitation Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学针灸学院,福州350122 [2]福建中医药大学中西医结合学院,福州350122 [3]福建中医药大学康复医学院,福州350122

出　　处：《针刺研究》2020年第8期623-627,共5页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81774385);国家自然科学基金青年科学基金项目(No.81704149);福建省自然科学基金项目(No.2016J01391);2017年度福建省高等学校新世纪优秀人才支持计划项目(No.闽教科[2017]52号)。

摘　　要：目的:观察腕踝针"下4"-"下5"-"下6"穴对坐骨神经分支选择性损伤(SNI)大鼠脊髓背角谷氨酸(Glu)及其N-甲基-D-天冬氨酸受体亚基1(NMDAR1)磷酸化蛋白表达的影响,探讨腕踝针改善坐骨神经痛的作用机制。方法:雄性SD大鼠随机分为假手术组、模型组、腕踝针组,每组12只。采用结扎并剪断坐骨神经中的腓总神经和胫神经、保留腓肠神经的方法制备SNI大鼠模型。腕踝针组于SNI后5~14 d予右侧"下4"-"下5"-"下6"穴腕踝针治疗,每天1次,每次4 h,连续10 d。采用von-Frey测痛仪记录大鼠机械痛阈值变化,丙酮记录冷异常性疼痛行为学变化;采用核磁共振氢谱、酶联免疫吸附法检测脊髓背角Glu的含量;采用免疫组织化学法检测右侧脊髓背角谷氨酸受体NMDAR1磷酸化蛋白的水平。结果:与假手术组相比,模型组大鼠机械痛阈值显著下降(P<0.01),冷刺激缩足持续时间明显增加(P<0.01),脊髓背角Glu含量及NMDAR1磷酸化蛋白表达显著增加(P<0.05,P<0.01);与模型组相比,腕踝针组大鼠机械痛阈值显著提高(P<0.01),冷刺激缩足持续时间明显缩短(P<0.01),脊髓背角Glu含量及NMDAR1磷酸化蛋白表达显著降低(P<0.05,P<0.01)。结论:腕踝针可减轻神经痛大鼠痛敏反应,其镇痛作用机制可能与抑制脊髓背角Glu及NMDAR1磷酸化蛋白表达有关。Objective To observe the effect of wrist-ankle acupuncture(WA)stimulation at"R4"-"R5"-"R6"on the expression of glutamate(Glu)and phosphorylated protein NMDAR1(p-NMDAR1)of the spinal dorsal horn in spared nerve injury(SNI)rats,so as to explore its mechanism underlying improvement of SNI.Methods A total of 36 SD rats were randomly divi-ded into sham operation,model and WA groups,with 12 rats in each group.The SNI procedure comprised an axotomy and ligation of the tibial and common peroneal nerves leaving the sural nerve intact.Rats of the WA group were treated by acupuncture at"R4"-"R5"-"R6"points from the 5th day to the 14th day after modeling.The mechanical pain thresholds were measured before and 5,10 and 14 d after SNI,respectively.The cold allodynia was dectected by Acetone solution dropped onto the lateral plantar surface of the paw.Glu content and p-NMDAR1 expression of spinal dorsal horn were detected by 1H-MRS,ELISA and immunohistochemistry methods.Results Compared with the sham operation group,the mechanical pain threshold of the model group was significantly decreased(P<0.01),the duration of cold stimulation foot contraction was increased(P<0.01),and the Glu content and p-NMDAR1 expression in the spinal dorsal horn were significantly increased(P<0.05,P<0.01).After WA intervention,the mechanical pain threshold was significantly increased(P<0.01),the duration of cold stimulation was significantly shortened(P<0.01),and Glu content and p-NMDAR1 protein expression of spinal dorsal horn were decreased significantly(P<0.05,P<0.01)in the WA group compared with the model group.Conclusion WA can reduce pain sensitivity in rats with neuropathic pain,possibly by inhibiting the expression of Glu and p-NMDAR1 in the spinal dorsal horn.

关 键 词：神经痛 腕踝针 脊髓背角 谷氨酸 N-甲基-D-天冬氨酸受体亚基1 

分 类 号：R245.31[医药卫生—针灸推拿学]