清醒无束缚大鼠在针刺缓解急性踝关节炎性痛实验中的应用  

Application of unrestrained conscious rats with acute inflammatory ankle pain to study of acupuncture analgesia

作　　者：郑雅文 吴明玥 沈雪勇[1,2] 汪丽娜 ZHENG Ya-wen;WU Ming-yue;SHEN Xue-yong;WANG Li-na(School of Acupuncture-moxibustion and Tuina,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Research Center for Acupuncture and Meridians,Shanghai 201203;Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function,Fudan University,Shanghai 201433)

机构地区：[1]上海中医药大学针灸推拿学院,上海201203 [2]上海市针灸经络研究中心,上海201203 [3]复旦大学上海市针灸机制与穴位功能重点实验室,上海201433

出　　处：《针刺研究》2020年第8期645-651,共7页Acupuncture Research

基　　金：国家自然科学基金项目(No.81574076);上海市中医药事业发展三年行动计划[No.ZY(2018-2020)-CCCX-2001-05];上海中医药大学杏林中青年人才培养计划。

摘　　要：目的:比较清醒无束缚方式和传统处理方式对大鼠针刺镇痛效应的影响差异,分析用自主行为指标评价针刺镇痛效应的可能性。方法:在比较不同处理方式的实验中,SD大鼠随机分为空白组、模型组、清醒无束缚针刺组、束缚针刺组、麻醉针刺组,每组6~9只。采用左侧踝关节注射完全弗氏佐剂(CFA)建立急性炎性痛模型。48 h后,各针刺组于同侧"足三里"处接受20 min针刺干预。分别于造模前、针刺治疗前和针刺治疗后30 min进行足底热和机械痛阈值测定。在自主行为测试实验中,SD大鼠随机分为空白组、模型组、清醒无束缚针刺组,每组11~12只。针刺方法同前。针刺结束后行旷场实验。结果:与空白组比较,CFA造模能显著降低各组大鼠机械痛阈值(P<0.001)和热痛阈值(P<0.001)。针刺干预后,与模型组比较,各针刺组大鼠的机械痛阈值和热痛阈值均明显升高(P<0.001,P<0.01);清醒无束缚针刺组机械痛阈值和热痛阈值明显高于束缚针刺组(P<0.05,P<0.01)和麻醉针刺组(P<0.01)。在旷场实验中,清醒无束缚针刺组大鼠爬壁次数较模型组显著增加(P<0.05),活动总路程和抑郁样行为虽有一定程度的改善,但与模型组比较,差异无统计学意义(P>0.05)。结论:和传统的束缚与麻醉比较,大鼠的清醒无束缚状态能更好地反映出针刺镇痛效应;和自主行为指标比较,痛阈能更好地反映出针刺镇痛效应。Objective To compare the analgesic effect of manual acupuncture(MA)stimulation of"Zusanli"(ST36)in rats with inflammatory pain under unrestrained conscious,restrained and general anesthesia conditions,so as to explore the applicability of unrestrained conscious model in the evaluation of acupuncture analgesia effect.Methods Male SD rats were divided into 5 groups:blank control(n=9),pain model(n=7),unrestrained conscious conditions+MA(n=6),restrained conditions+MA(n=6),and general anesthesia(GA)+MA(n=6).The acute pain model was established by injection of complete Freund’s adjuvant(CFA)into the left ankle joint 48 h ahead of acupuncture.Subsequently,a single 20 min session of MA was applied to the left ST36.The mechanical and thermal pain thresholds(MPT and TPT)were determined before and after injection of CFA,and after MA stimulation.In order to evaluate the autonomic behavior activities,rats were randomly divided into blank control(n=11),pain model(n=11)and conscious-unrestrained conditions+MA(n=12)groups.The rats’exploratory movements were assessed by open field tests.Results Both MPT and TPT were significantly decreased after injection of CFA in the model group relevant to the blank control group(P<0.001),and significantly higher in the three MA groups than in the model group(P<0.001).Comparison among the three MA groups showed that both MPT and TPT were significantly higher in the conscious unrestrained conditions+MA group than in the restrained conditions+MA and GA+MA groups(P<0.05,P<0.01).Open filed tests showed that the total moving distance in the open field and wall climbing times were significantly lower in the model group than in the blank control group(P<0.01),and the wall climbing times were obviously more in the unstrained conditions+MA group than in the model group(P<0.05).The central area resistance time was significantly shorter in the model group than in the control group(P<0.05),and was moderately increased after MA despite no evident significance(P>0.05).No significant changes were found i

关 键 词：清醒无束缚模型 针刺镇痛 痛阈 自主行为 

分 类 号：R245.0[医药卫生—针灸推拿学]