基于纳米材料的骨靶向光热治疗技术在转移性骨肿瘤治疗中的应用及预见性护理  

Application and predictive nursing of bone-targeting photothermal therapy based on Nanomaterials in the treatment of metastatic bone tumors

作　　者：徐巧巧 许洪 XU Qiaoqiao;XU Hong(Affiliated Hospital of Hubei University of Arts and Science,Xiangyang Central Hospital,Hubei 441021 China)

机构地区：[1]湖北文理学院附属医院襄阳市中心医院,湖北441021

出　　处：《护理研究》2020年第17期3026-3033,共8页Chinese Nursing Research

摘　　要：[目的]探讨基于纳米材料的骨靶向光热治疗技术在转移性骨肿瘤治疗中应用效果及预见性护理。[方法]分别制造合成末端基团不同的树形高分子铂光热纳米材料G5-NH2-AC-PT、G4.5-COOH-Pt、G5-NH2-Pt,并结合裸鼠生物实验来验证不同纳米材料的表征指标、骨靶向性能和裸鼠的生物性实验疗效。[结果]通过实验得到,3种纳米光热材料具有相似的形貌和均一尺寸,3种光热纳米材料的直径12 nm,水合直径在10 nm左右。在近红外光照射作用下,G4.5-COOH-Pt的光热效果最好。通过裸鼠体内光热胫骨肿瘤实验可以看出,G4.5-COOH+NIR组的治疗效果要显著优于其他组,差异具有统计学意义(P<0.05)。[结论]合成制备的G5-NH2-AC-PT、G4.5-COOH-Pt、G5-NH2-Pt都具有较好的光热治疗效果,但是G4.5-COOH-Pt材料对肿瘤的杀伤效果更为显著,有着更好的骨破坏抑制作用。Objective:To explore the application effects of bone-targeting photothermal therapy based on nanoparticles in treatment of metastatic bone tumors and possible predictive nursing.Methods:Firstly,different platinum-doped polyamidoamine(PAMAM)dendrimers photothermal nanoparticles with an acetylated amino-terminal(generation5. 0,G5-NH2-AC-Pt),a carboxyl-terminal(generation 4. 5,G4. 5-COOH-Pt)and an amino-terminal(generation5. 0,G5-NH2-Pt)were synthesized,respectively. Then,combined with bioexperiments in nude mice,the characteristic indexes,bone targeting properties and treatment efficacy in bioexperiments were verified among different nanoparticles.Results:The experiments results showed that the three kinds of composite photothermal nanoparticles had similar morphology and uniform size. The diameters of three photothermal nanoparticles were 1-2 nm. And the hydration diameters were about 10 nm. Under the irradiation effect of near infrared light(NIR),photothermal effect of G4. 5-COOH-Pt was the best. Through the photothermal tibia tumor experiment in nude mice,it could be seen that the therapeutic effect of G4. 5-COOH combined with NIR group was significantly better than those of other four groups. The difference was statistically significant(P<0. 05).Conclusions:The synthesized PAMAM dendrimers G5-NH2-AC-PT,G4. 5-COOH-Pt and G5-NH2-Pt had better photothermal therapeutic effect. In addition,G4. 5-COOH-Pt nanoparticles had more significant effects on killing tumor cells and inhibiting destruction of bone.

关 键 词：光热治疗 骨肿瘤 骨靶向 纳米材料 转移瘤 预见性护理 

分 类 号：R473.73[医药卫生—护理学]