全自动免疫组化仪与第一抗体不匹配导致假阳性分析  

A False Positive Analysis on the Mismatch Between the Automatic Immunohistochemistry Instrument and the First Antibody

作　　者：郭凯[1] 张金秋 梁洁 陈海霞 印永祥 GUO Kai;ZHANG Jinqiu;LIANG Jie;CHEN Haixia;YIN Yongxiang(Department of Pathology,Suzhou Hospital Affiliated to Nanjing Medical University,Suzhou Science and TechnologyTown Hospital,Suzhou 215153, China;Department of Pathology, the Affiliated Maternity and Child HealthHospital of Nanjing Medical University, Wuxi 214002, China)

机构地区：[1]南京医科大学附属苏州医院,苏州科技城医院病理科,江苏苏州215153 [2]南京医科大学附属无锡妇幼保健院病理科,江苏无锡214002

出　　处：《标记免疫分析与临床》2020年第8期1409-1411,共3页Labeled Immunoassays and Clinical Medicine

基　　金：无锡市卫生计生委“科教强卫工程”项目(编号:ZDRCPY006)。

摘　　要：目的通过免疫组织化学(immunohistochemistry,IHC)对肿瘤细胞进行正确的免疫表型分析和精准组织病理学诊断。方法采用非生物素两步法和全自动免疫组化染色仪进行免疫组化染色,显示CD20在肿瘤组织中表达。结果全自动免疫组化仪与第一抗体不匹配导致假阳性的发生。结论在诊断免疫组织化学中使用抗体和全自动免疫组化仪自体配套的二抗试剂盒的相容组合非常重要。A correct immunophenotype analysis of tumor cells by immunohistochemistry(IHC)is essential for the accurate histopathological diagnosis.The application of a fully automatic immunohistochemical staining instrument has promoted the standardization and quality control of immunohistochemistry.In this article,we report the occurrence of false positives caused by a mismatch between the automatic immunohistochemistry instrument and the primary antibody,and illustrate the importance of using compatible combinations of antibodies and the secondary antibody kits provided with the automatic immunohistochemistry instrument in diagnostic immunohistochemistry.

关 键 词：CD20 抗原修复 全自动免疫组织化学 假阳性 

分 类 号：R446.8[医药卫生—诊断学]