女性供者特征对粒细胞集落刺激因子动员的骨髓和外周血混合采集物中造血和免疫细胞组份的影响  

Effects of female healthy donor characteristics on CD34^+cells and immune cells in the mixed collection of bone marrow and peripheral blood mobilized by granulocyte colony-stimulating factor

作　　者：尚博菲 孙于谦[1] 王欣玉 常英军[1] 刘艳荣[1] 王昱[1] 黄晓军[1] 赵晓甦[1] Shang Bofei;Sun Yuqian;Wang Xinyu;Chang Yingjun;Liu Yanrong;Wang Yu;Huang Xiaojun;Zhao Xiaosu(Peking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, China)

机构地区：[1]北京大学人民医院,北京大学血液病研究所,血液系统疾病国家临床医学研究中心,造血干细胞移植治疗血液病北京市重点实验室,北京100044

出　　处：《临床荟萃》2020年第10期904-908,共5页Clinical Focus

基　　金：国家自然科学基金资助项目——针对新靶点microRNA-153治疗急性移植物抗宿主病的分子机制研究(81670175);国家自然科学基金资助项目——粘膜相关恒定T细胞调控造血干细胞移植后肠道移植物抗宿主病发生的机制研究(81870137)。

摘　　要：目的探讨女性健康供者特征对粒细胞集落刺激因子(G-CSF)预激的骨髓和外周血混合采集物造血细胞和免疫细胞组份的影响。方法111名健康女性供者应用G-CSF动员,用流式细胞仪测定骨髓和外周血混合采集物中的CD34^+细胞和T细胞亚群的数量,并分析怀孕、年龄、身高等供者特征对骨髓和外周血采集物中细胞组份的影响。结果111例女性健康供者骨髓和外周血混合采集物中的CD34^+细胞、CD3^+T细胞、CD3^+CD4^+T细胞、CD3^+CD8+T细胞和CD3^+CD4^-CD8^-调节性T细胞的数量以每公斤供者体重计算的中位值分别为2.39×106/kg、226.57×106/kg、120.80×106/kg、89.99×106/kg和15.05×106/kg。①年龄对混合移植物中CD3^+CD4^-CD8^-调节性T细胞的数量有影响。②体重指数(BMI)对混合采集物中的CD3^+T细胞以及CD3^+CD4^-CD8^-T细胞的数量有影响;③外周血干细胞采集当天的淋巴细胞(LYM)对混合采集物中的CD3^+T细胞、CD3^+CD4^+T细胞和CD3^+CD8+T细胞的数量有影响;④骨髓干细胞采集当天的LYM对混合采集物中CD3^+T细胞和CD3^+CD4^-CD8^-调节性T细胞的数量有影响;⑤与怀孕供者相比,未怀孕供者混合采集物中含有更高数量的CD3^+CD8+T细胞。结论供者年龄、BMI、是否怀孕以及骨髓干细胞和外周血干细胞采集当天的LYM是骨髓免疫组份的主要影响因素。Objective To investigate the effects of female healthy donor characteristics on the components of hematopoietic cells and immune cells from a mixture of bone marrow and peripheral blood collection preactivated by granulocyte colony-stimulating factor(G-CSF).Methods A total of 111 healthy female donors were mobilized with G-CSF to determine the number of CD34^+cells and T-cell subsets in a mixture of bone marrow and peripheral blood by flow cytometry.The effects of pregnancy,age,height and other donor characteristics on the cell components in bone marrow and peripheral blood collection were analyzed.Results The median values of CD34^+cells,CD3^+T cells,CD3^+CD4^+T cells,CD3^+CD8+T cells and CD3^+CD4^-CD8^-regulatory T cells in the mixture of bone marrow and peripheral blood samples from 111 healthy female donors were 2.39×106,226.57×106,120.80×106,89.99×106 and 15.05×106 per kg of donor body weight,respectively.①Age had an effect on the number of CD3^+CD4^-CD8^-T cells in the mixed allografts.②Body mass index(BMI)had an effect on the number of CD3^+T cells and CD3^+CD4^-CD8^-T cells in the mixed collection.③The number of CD3^+T cells,CD3^+CD4^+T cells and CD3^+CD8+T cells in the mixture was affected by lymphocyte(LYM)counts on the day of peripheral blood stem cell collection.④The number of CD3^+T cells and CD3^+CD4^-CD8^-T cells in the mixture was affected by lymphocyte counts on the day of bone marrow stem cell collection.⑤Compared with pregnant donors,the mixed collection of non pregnant donors contained a higher number of CD3^+CD8+T cells.Conclusion Donor age,BMI,pregnancy status and LYM on the day of bone marrow stem cells and peripheral blood stem cells were the main influencing factors of bone marrow immunohistochemical components.

关 键 词：造血干细胞移植 粒细胞集落刺激因子 活体供者 体重指数 移植免疫学 

分 类 号：R617[医药卫生—外科学]