吡虫啉在三种芸薹属蔬菜中的残留行为及风险评估  

Study on Residue Behavior and Risk Assessment of Imidacloprid in Three Brassica Vegetables

作　　者：董见南 吕洋 刘峰 陈国峰 张晓波 廖辉 秦冬梅 Dong Jiannan;Liu Feng;Chen Guofeng;Zhang Xiaobo;Liao hui;Lü Yang;Qin Dongmei(Safety and Quality Institute of Agricultural Products,Heilongjiang Academy of Agricultural sciences,Harbin Heilongjiang 150086,China;Institute of Mudanjiang,Heilongjiang Academy of Agricultural Sciences,Mudanjiang Heilongjiang 157000,China;Institute for the Control of Agrochemicals,Ministry of Agriculture and Rural Affairs,Beijing 100125,China)

机构地区：[1]黑龙江省农业科学院农产品质量安全研究所,黑龙江哈尔滨150086 [2]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157000 [3]农业农村部农药检定所,北京100125

出　　处：《农药科学与管理》2020年第8期26-32,共7页Pesticide Science and Administration

基　　金：黑龙江省农业科学院2017年度院级科研项目(2017BZ15);农业农村部农业行业标准制定与修订项目。

摘　　要：建立了分析菜薹、芥蓝和青花菜中吡虫啉残留量的方法。样品经乙腈震荡提取,盐析后取乙腈相浓缩,PSA和GCB分散固相萃取净化后,高效液相色谱-串联质谱法检测。吡虫啉在3种蔬菜中的平均回收率分别为82.2%~99.7%、92.9%~108.3%和80.3%~100.5%,相对标准偏差分别为2.5%~6.0%、0.6%~3.1%和1.9%~2.8%,定量限均为0.02mg/kg。在黑龙江省、江苏省和湖南省进行田间试验,按照20%吡虫啉可溶液剂的推荐施药方法施药,末次施药5、7、10d后,菜薹中吡虫啉的残留量为<0.02~0.080mg/kg,芥蓝中吡虫啉的残留量为<0.02~0.11mg/kg,青花菜中吡虫啉的残留量为<0.02~0.33mg/kg。经长期膳食摄入风险评估,我国普通人群吡虫啉的日摄入风险概率最大为11.7%,建议制定吡虫啉在菜薹、芥蓝和青花菜中的最大残留限量分别为0.5mg/kg、1.0mg/kg和1.0mg/kg。A method of determination of imidacloprid residue in brassica parachinensis bailey,brassica alboglabra bailey and broccoli was developed.The samples were extracted with acetonitrile and determined by HPLC-MS/MS after being concentrated and cleaned up by dispersive solid phase extraction with PSA and GCB.The recoveries of imidacloprid in brassica parachinensis bailey,brassica alboglabra bailey and broccoli,plant and soil were 82.2%~99.7%,92.9%~108.3%,80.3%~100.5%and 81.5%~99.1%,with the relative standard deviations of 2.5%~6.0%,0.6%~3.1%and 1.9%~2.8%,respectively.The minimum detectable concentrations were all 0.02 mg/kg in the three varieties of vegetable.The field trials were carried out in Heilongjiang,Jiangsu and Hunan province.Imidacloprid 20%soluble concentrate were sprayed at the recommended doses,and the samples were collected after 5,7,10 days of the last spraying.The terminal residues of imidacloprid in brassica parachinensis bailey were<0.02~0.080 mg/kg,<0.02~0.11 mg/kg in brassica alboglabra bailey,and<0.02~0.33 mg/kg in broccoli.After long-term dietary intake risk assessment,the daily risk probability in the general population was 11.7%at most.Therefore,it is suggested that the maximum residue limits of imidacloprid in brassica parachinensis bailey,brassica alboglabra bailey and broccoli should be 0.5 mg/kg,1.0 mg/kg and 1.0 mg/kg,respectively.

关 键 词：吡虫啉 芸薹属蔬菜 残留 风险评估 

分 类 号：S482.3[农业科学—农药学] S481.8[农业科学—植物保护]