一株红假单胞菌的分离及对Cu2+的去除  

Isolation and identification of a copper-removal Rhodopseudomonas strain

作　　者：张晓倩 杨阔 王宁[1] 赵敏[1] 汪春蕾[1] ZHANG Xiao-Qian;YANG Kuo;WANG Ning;ZHAO Min;WANG Chun-Lei(Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education/College of Life Sciences,Northeast Forestry University,Harbin,Heilongjiang 150040,China)

机构地区：[1]东北林业大学生命科学学院/东北盐碱植被恢复与重建教育部重点实验室,黑龙江哈尔滨150040

出　　处：《微生物学通报》2020年第8期2392-2398,共7页Microbiology China

基　　金：国家自然科学基金(51678120)。

摘　　要：【背景】重金属污染对环境和人类的健康构成了重大威胁,因此,重金属污染的治理迫在眉睫。生物治理因成本低、处理效果好和无二次污染等优点,在处理重金属污染时被优先选择。【目的】从辽河入海口深13 m的水体中,利用紫色非硫细菌富集培养基筛选产色素能力强并对高浓度Cu2+有高效去除能力的菌株。【方法】采用形态学、生理生化特性和分子生物学方法鉴定菌种;采用二乙基二硫代氨基甲酸钠分光光度法测定Cu2+的含量。【结果】鉴定菌株为红假单胞菌属,将其命名为Rhodopseudomonas sp.gh32。菌株gh32的最适生长温度和最适pH分别为30°C和7.0,其在pH 5.0-10.0范围内可正常生长,在3 mmol/L的CuSO4溶液中能够正常生长,能利用葡萄糖、甘露糖和果糖等单糖和硫化氢。菌株gh32在24h内对Cu2+的去除率均在99%以上,处理能力为1331 g/g干菌重或167.6 g/g湿菌重。【结论】菌株gh32对Cu2+具有较强的耐受性和很好的去除效果,是治理含Cu2+废水的潜力菌株。本研究为生物治理重金属废水提供了支持。[Background] The treatment of heavy metal pollution is imminent because heavy metal poses a major threat to the environment and human health. Biological treatment methods are preferred because of the advantages of low cost, good treatment effect and no secondary pollution. [Objective] From the 13 m deep water body of the Liaohe estuary, a strain possessing strong pigment-producing ability and highconcentration Cu2+ removal ability was screened by the purple non-sulfur bacteria enrichment medium. [Methods] Morphological, physiological and biochemical characteristics and molecular biological methods were used to identify the strain. The content of Cu2+ was determined by spectrophotometry with sodium diethyldithiocarbamate. [Results] The strain was identified as Rhodopseudomonas and named as Rhodopseudomonas sp. gh32. The optimal growth temperature and growth pH of strain gh32 were 30 °C and 7.0, respectively. The strain gh32 grew normally in the range of pH 5.0-10.0. The strain gh32 could grow normally in 3 mmol/L CuSO4 solution. The strain gh32 could utilize hydrogen sulfide and monosaccharides, such as glucose and mannose. The removal rate of Cu2+ was over 99% in strain gh32 within 24 h. The ability to treat Cu2+ was 1 331 g/g dry bacterial weight or 167.6 g/g wet bacterial weight. [Conclusion] The strain gh32 was highly resistant to Cu2+ and there was a high removal rate of Cu2+ by strain gh32. It will be a potential strain of treatment wastewater containing Cu2+. This study provided support for biological treatment of heavy metal wastewater.

关 键 词：菌种鉴定 红假单胞菌 Cu2+去除 

分 类 号：X703[环境科学与工程—环境工程] X172