泓森槐可培养内生固氮细菌多样性与潜在促生长特性评价  

Evaluating diversity and potential growth promoting characteristics of the culturable endophytic diazotrophic bacteria isolated from Robinia pseudoacacia ’Hongsen’

作　　者：付思远 席雨晴 赵鹏菲 梁永健 宋旭 常华瑜 彭桂香[2] 谭志远[1] FU Si-Yuan;XI Yu-Qing;ZHAO Peng-Fei;LIANG Yong-Jian;SONG Xu;CHANG Hua-Yu;PENG Gui-Xiang;TAN Zhi-Yuan(College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642,China;College of Resource and Environment,South China Agricultural University,Guangzhou,Guangdong 510642,China)

机构地区：[1]华南农业大学农学院,广东广州510642 [2]华南农业大学资源环境学院,广东广州510642

出　　处：《微生物学通报》2020年第8期2458-2470,共13页Microbiology China

基　　金：国家自然科学基金(31970001);广东省科技计划(2018A050506075,2018B020205003,2018B020206001);广东省植物分子育种重点实验室开放基金。

摘　　要：【背景】泓森槐为豆科刺槐属的速生型落叶乔木,生长快,营养需求多。目前对于泓森槐内生固氮细菌的研究鲜有报道。【目的】研究泓森槐可培养内生固氮细菌的多样性及潜在促生长特性。【方法】从表面消毒的泓森槐根、茎、叶及根瘤中进行内生固氮细菌的分离纯化,采用IS-PCR指纹图谱技术将分离得到的菌株进行聚类,对各类群代表菌株进行16SrRNA基因系统发育分析、生理生化试验、固氮酶活性测定、促生长特性试验。【结果】从泓森槐中分离出56株细菌,聚为10个类群,分别为Paenibacillus sabinae、Klebsiella michiganensis、Kosakonia radicincitans、Kosakonia pseudosacchari、Mesorhizobium erdmanii、Mesorhizobium huakuii、Mesorhizobium silamurunense、Pseudomonas geniculata、Burkholderia territorii和Devosia riboflavina,其中5个类群为联合固氮菌、3个类群为共生固氮菌、2个类群为非固氮菌,表明泓森槐组织内生固氮细菌具有丰富的多样性。促生特性试验表明7个类群具溶磷能力、6个类群具解钾能力、6个类群具有产生长素能力、6个类群具产铁载体能力、2个类群具有产蛋白酶能力。【结论】泓森槐组织内生固氮细菌具有遗传多样性与促生长特性,其在农业微生物菌肥开发利用方面具有潜在价值。[Background] Robinia pseudoacacia ‘Hongsen’ is a fast-growing deciduous tree of Robinia in Leguminosae. Endophytic diazotrophic bacteria of Robinia pseudoacacia ‘Hongsen’ is being researched little nowadays. [Objective] We studied the diversity and growth promoting characteristics of the culturable endophytic diazotrophic bacteria isolated from Robinia pseudoacacia ‘Hongsen’. [Methods] Strains were isolated and purified from surface sterilized roots, stems, leaves and root nodules of Robinia pseudoacacia ‘Hongsen’, the isolates were clustered by using IS-PCR finger-printing, nitrogenase activity of the representative strain of each group was measured, we also conducted phylogenetic analysis of 16 S rRNA gene, physiological and biochemical tests and growth promoting experiments of the representative strains. [Results] A total of 56 strains were isolated from Robinia pseudoacacia ‘Hongsen’, belonging to ten groups: Paenibacillus sabinae, Klebsiella michiganens, Kosakonia radicincitans, Kosakonia pseudosacchari, Mesorhizobium erdmanii, Mesorhizobium huakuii, Mesorhizobium silamurunense, Pseudomonas geniculate, Burkholderia territorii and Devosia riboflavina, in which 5 groups were associative nitrogen-fixing bacteria, 3 groups were symbiotic nitrogen-fixing bacteria, 2 groups had not the nitrogen-fixing ability, which showed the rich diversity of endophytic diazotrophic bacteria of Robinia pseudoacacia ‘Hongsen’. Growth promoting experiments showed that 7 groups had the function of phosphorus solubility, 6 potassium solubility, 6 auxin secretion, 6 siderophore production, and 2 proteases production. [Conclusion] Endophytic diazotrophic bacteria isolated from Robinia pseudoacacia ‘Hongsen’ have abundant genetic diversity and growth promoting characteristics, which have potential value in exploitation and utilization of agricultural microbial fertilizer.

关 键 词：泓森槐 内生菌 固氮菌 促生长特性 

分 类 号：S792.99[农业科学—林木遗传育种]