四君子颗粒含药血清对棕榈酸诱导的MIN6细胞凋亡的影响  

Effects of Sijunzi Granules medicated serum on MIN6 cell apoptosis induced by palmitic acid

作　　者：唐保露 郑宇辰 张叶明 袁萍 戚小宇 张旭[1] 杨解人[1] 郑书国[1,4] TANG Bao-lu;ZHENG Yu-chen;ZHANG Ye-ming;YUAN Ping;QI Xiao-yu;ZHANG Xu;YANG Jie-ren;ZHENG Shu-guo(Department of Pharmacology,Wannan Medical College,Wuhu 241002,China;College of Clinical Medicine,Wannan Medical College,Wuhu 241002,China;College of Pharmacy,Wannan Medical College,Wuhu 241002,China;Institute of Quantitative Pharmacology,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院药理学教研室,安徽芜湖241002 [2]皖南医学院临床医学院,安徽芜湖241002 [3]皖南医学院药学院,安徽芜湖241002 [4]皖南医学院定量药理研究所,安徽芜湖241002

出　　处：《中成药》2020年第8期2031-2036,共6页Chinese Traditional Patent Medicine

基　　金：安徽省高校省级自然科学研究重点项目(KJ2015A192);中国医药教育协会孙思邈中医药科研专项课题(2016SKT-M033);皖南医学院重点科研项目培育基金(WK2018Z05);国家级大学生创新创业训练计划项目(201910368012)。

摘　　要：目的探讨四君子颗粒含药血清对棕榈酸诱导的MIN6细胞凋亡的影响。方法SD大鼠灌胃给予四君子颗粒(10.5、5.25 g/kg)7 d,制备四君子颗粒含药血清。四君子颗粒含药血清预处理MIN6细胞24 h后,加入0.4 mmol/L棕榈酸孵育MIN6细胞24 h。CCK-8法检测细胞活力,流式细胞术检测细胞凋亡,Western blot法检测GRP78、ATF4、CHOP、p-PERK蛋白表达。结果四君子颗粒含药血清减轻了棕榈酸诱导的MIN6细胞损伤和凋亡(P<0.05,P<0.01),下调内质网应激相关蛋白GRP78、ATF4、CHOP蛋白表达,降低PERK蛋白磷酸化(P<0.05,P<0.01)。结论四君子颗粒含药血清可有效减轻棕榈酸诱导的MIN6细胞损伤和凋亡,其机制可能与抑制内质网应激相关的信号通路有关。AIM To investigate the effects of Sijunzi Granules(SGs)medicated serum on MIN6 cell apoptosis induced by palmitic acid.METHODS SD rats were given 7-day intragastric SGs administration(10.5,5.25 g/kg)for preparation of SGs medicated serum.MIN6 cells pretreated with 24 h SGs serum were then exposed to 0.4 mmol/L palmitic acid induction for 24 h.The MIN6 cells were subsequently subjected to the detection of cell viability by CCK-8 method,investigation of cell apoptosis by flow cytometry,and detection of GRP78,ATF4,CHOP,and p-PERK protein expression by Western blot.RESULTS SGs medicated serum alleviated injury and apoptosis of palmitic acid-induced MIN6 cells(P<0.05,P<0.01),and down-regulated the expression of endoplasmic reticulum stress-related proteins GRP78,ATF4,and CHOP expression,and reduced PERK protein phosphorylation as well(P<0.05,P<0.01).CONCLUSION SGs medicated serum can effectively reduce MIN6 cells injury and apoptosis induced by palmitic acid-induced,and its mechanism may be related to the inhibition of endoplasmic reticulum stress-related signaling pathways.

关 键 词：四君子颗粒 细胞凋亡 内质网应激 糖尿病 

分 类 号：R285.5[医药卫生—中药学]