外源壳寡糖提高鲁梅克斯对铜胁迫的耐受性研究  

Study on the Effect of Exogenous Chitooligosaccharides on the Tolerance of Rumax to Copper Stress

作　　者：王丽娟[1] 于星宇 冯旭[1] 李凤兰[1] 胡宝忠[1] WANG Lijuan;YU Xingyu;FENG Xu;LI Fenglan;HU Baozhong(College of Life Science,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业科学》2020年第3期60-64,共5页Journal of Northeast Agricultural Sciences

基　　金：国家国际科技合作专项(2013DFR30270);黑龙江省应用技术研究与开发技术新创办企业项目(GC15F010)。

摘　　要：铜元素对植物的生长至关重要,但土壤中过量的铜不但影响农作物的产量和品质,还会通过食物链威胁到动物及人类的健康。因此,寻找有效缓解铜胁迫对植物生长的抑制及降低植物中铜含量的方法对农业生产有重要意义。本研究通过盆栽试验探讨了外源施加不同浓度的壳寡糖对铜胁迫下鲁梅克斯生长、抗氧化系统及对铜元素富集和转运的影响,为铜污染土地中农作物的种植提供新的策略。结果表明:外源壳寡糖显著提高了铜胁迫下鲁梅克斯的叶绿素含量和抗氧化酶SOD、POD及CAT的活性,并且降低了植株对铜的富集及转运系数,进而提高了鲁梅克斯对铜胁迫的耐受性并降低了茎叶中的铜含量。壳寡糖的施用浓度要控制在合理的水平,50~100 mg/L的浓度较为适宜。Copper is very important to plant growth, but excessive copper in soil not only affects the yield and quality of crops, but also threatens the health of animals and humans through the food chain. Therefore, it is important for agricultural production to find effective ways to alleviate the inhibition of copper stress on plant growth and reduce copper content in plants. In this study, pot experiments were conducted to investigate the effects of different concentrations of chitooligosaccharide on Rumex growth, antioxidant system and copper enrichment and transport under copper stress, providing a new strategy for crop cultivation in copper-contaminated soil. The results showed that exogenous chitooligosaccharide significantly increased chlorophyll content and antioxidant enzymes activities of SOD,POD and CAT under copper stress, and reduced the accumulation and transport coefficients of copper, thus improving the tolerance of Rumex to copper stress and reducing copper content in stems and leaves. The application concentration of chitooligosaccharide should be controlled at a reasonable level, and the concentration of 50~100 mg/L is more suitable.

关 键 词：鲁梅克斯 铜胁迫 壳寡糖(COS) 耐受性 

分 类 号：S548[农业科学—作物学] Q945.78[生物学—植物学]