响应面优化红蓝草总酚酸的提取及其抗氧化活性研究  

Optimization of Extraction Process of Total Phenolic Acids from the Peristrophe Baphica(Spreng.)Bremek by Response Surface Methodology and Its Antioxidation

作　　者：韦正 赖红芳 彭丽娟 覃国乐 黄秀香 WEI Zheng;LAI Hong-fang;PENG Li-juan;QIN Guo-le;HUANG Xiu-xiang(College of Chemistry and Bio-Engineering,Hechi University,Yizhou 546300,Guangxi China)

机构地区：[1]河池学院化学与生物工程学院,广西宜州546300

出　　处：《食品研究与开发》2020年第18期84-90,共7页Food Research and Development

基　　金：广西自然科学基金项目(2017GXNSFAA198118);广西教育厅高校科学技术研究项目(KY2015YB265);红水河流域药用资源综合开发利用重点实验室项目;河池学院2018年高层次人才科研启动费项目(XJ2018GKQ012)。

摘　　要：对红蓝草总酚酸的提取及其抗氧化活性进行研究。结合单因素考察结果,用响应面法优选红蓝草总酚酸的最佳提取条件,即液料比为28∶1(mL/g),乙醇的浓度为71%,提取温度为70℃,提取时间为90 min,在该条件下红蓝草总酚酸含量为31.574 4 mg/g,与预测值31.860 6 mg/g接近。红蓝草总酚酸体外抗氧化活性评价中,发现其随着浓度增加,其清除能力逐渐增强,但清除率小于Vc。因此,红蓝草总酚酸有一定的抗氧化活性。This study mainly discussed the extraction technology of total phenolic acids from the Peristrophe baphica(Spreng)Bremek(PB)and its antioxidation. Combining with the single factor investigation results,and the optimum extraction condition of total phenolic acids from the PB was determined by the response surface methodology,such as solution and material ratio for 28 ∶ 1(mL/g),ethanol concentration for 71%,extraction temperature for 70 ℃,extraction time for 90 min,and extraction yield of total phenolic acids from the PB reached 31.574 4 mg/g at this condition,and the predictive value of 31.860 6 mg/g. The total phenolic acids in vitro antioxidant activity evaluation,found with the increase of concentration,its removal ability strengthens gradually,but clearance was less than Vc. The total phenolic acids from the PB exhibits certain antioxidant capacity.

关 键 词：红蓝草 总酚酸 提取 抗氧化 响应面 

分 类 号：TQ28[化学工程—有机化工]