MAE-HPLC-HG-AFS同时测定洛克沙砷及其8种代谢物  

Simultaneous Determination of Roxarsone and Its 8 Kinds of Metabolites by MAE-HPLC-HG-AFS

作　　者：崔颖 姜涛 倪松 逯玉凤 CUI Ying;JIANG Tao;NI Song;LU Yu-feng(Animal&Plant and Food Inspection Center of Tianjin Custom,Tianjin 300461,China;Beijing Haiguang Instruments Co.,Ltd.,Beijing 100015,China)

机构地区：[1]天津海关动植物与食品检测中心,天津300461 [2]北京海光仪器有限公司,北京100015

出　　处：《食品研究与开发》2020年第18期182-188,共7页Food Research and Development

基　　金：国家质量监督检验检疫总局科技计划项目(2017IK093)。

摘　　要：建立一种同时测定动物食品中洛克沙砷(roxarsone,ROX)及其8种代谢物的高效液相色谱-氢化物发生-原子荧光光谱法(high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry,HPLC-HGAFS)。对液相色谱、氢化物发生、原子荧光光谱以及提取条件进行优化。在0~240μg/L范围内ROX及其8种代谢物的线性关系良好,线性相关系数均大于0.997。4种样品的添加回收率在80.1%~96.0%范围内,相应的相对标准偏差(relative standard deviation,RSD)在9%以内。该方法适用于鸡肝、鸡肾、猪肝、猪肾样品中洛克沙砷及其8种代谢物的含量分析。A method for simultaneous determination of roxarsone(ROX)and its metabolites in foods of animal origin was developed by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry(HPLC-HG-AFS). The operating conditions of high performance liquid chromatography,hydride generation,atomic fluorescence spectrometry and extraction were optimized. Under the optimal conditions,the calibration curves for ROX and its metabolites were linear over the concentration ranges of 0-240 μg/L with the correlation coefficients were greater than 0.997. The average recoveries of ROX and its metabolites from four samples spiked were range of 80.1 %-96.0 %,respectively and their corresponding relative standard deviations(RSD)were less than 9 %. This method was suitable for the determination of roxarsone and its 8 metabolites in chicken liver,chicken kidney,pig liver and pig kidney.

关 键 词：高效液相色谱 氢化物发生 原子荧光光谱法 微波辅助萃取 洛克沙砷 代谢物 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]