基于生物金属有机骨架封装亚甲基蓝复合材料和信号放大策略高灵敏检测microRNA-141  

Biosensor Based on Methylene Blue Encapsulated Metal OrganicFramework and Signal Amplification Strategyfor Sensitive Detection of MicroRNA-141

作　　者：王存 刘蕃鑫 惠俊敏 邹晓川 邓明川 郑天平 刘林 WANG Cun;LIU Fan-Xin;HUI Jun-Min;ZOU Xiao-Chuan;DENG Ming-Chuan;ZHENG Tian-Ping;LIU Lin(Innovation Center for Lipid Resource and Children′s Daily Chemicals,College of Biological and Chemical Engineering,Chongqing University of Education,Chongqing 400067,China)

机构地区：[1]重庆第二师范学院脂质资源与儿童日化品协同创新中心,重庆400067

出　　处：《分析化学》2020年第9期1185-1192,共8页Chinese Journal of Analytical Chemistry

基　　金：重庆市教委科技研究项目(No.KJQN201901604);重庆市自然科学基金项目(No.cstc2018jcyjAX0110)资助。

摘　　要：MicroRNA-141(miRNA-141)的检测对相关癌症的早期诊断和预后评估具有重要意义。本研究建立了一种无酶的生物传感器,用于高灵敏检测miRNA-141。首先,合成了具有多孔结构和良好生物相容性的生物金属有机骨架(bio-MOF),通过离子交换的方式,bio-MOF可以封装大量亚甲基蓝(MB)信号分子,并保持其生物活性。其次,利用目标物循环信号放大策略中的催化发夹自组装(CHA)、辅助目标miRNA-141循环,实现信号放大,进一步提高了传感器的灵敏度。在1.0×10^16~1.0×10^6 mol/L范围内,目标miRNA-141浓度的对数与传感器电化学信号呈线性相关,检出限为3.3×10^17 mol/L(S/N=3)。将此方法应用于健康人血清中miRNA-141检测,加标回收率为99.9%~102.0%(RSD≤2.1%)。此生物传感器简单、可靠、灵敏度高、线性范围宽,为高灵敏检测其它miRNA提供了参考。MicroRNA-141(miRNA-141)is of great significance in the early diagnosis and treatment of related cancers.Here,a biosensor was developed for detection of miRNA-141.Firstly,bio-metal organic framework(bio-MOF)with a certain porosity and good biocompatibility was synthesized.The bio-MOF could encapsulate a large number of methylene blue(MB)via ion-exchange process and maintain the biological activity of MB.Secondly,as one of the target recycling signal amplification technologies,catalyzed hairpin assembly(CHA)could assist the target miRNA-141 cycle to achieve signal amplification and further improve the biosensor sensitivity.As a result,the proposed ECL biosensor exhibited good sensitivity for microRNA-141 detection in a linear range from 1.0×10^16 mol/L to 1.0×10^6 mol/L with a limit of detection(LOD)of 3.3×10^17 mol/L(S/N=3).The spiked recoveries ranged from 99.9%to 102.0%(RSD≤2.1%).The biosensor showed good stability and selectivity,wide linear range and high sensitivity,and opened up a new avenue for highly sensitive detection of various miRNAs.

关 键 词：生物传感器 催化发夹自组装 信号放大策略 MicroRNA-141 生物金属有机骨架 亚甲基蓝 

分 类 号：R318.08[医药卫生—生物医学工程] TP212[医药卫生—基础医学]