基于显色法的纸基微流控芯片用于水中的三氮检测  

Paper-based Microfluidic Chip for Detection of 3-Nitrogen in Water Based on Chromogenic Method

作　　者：徐榕 杨叶欣 林敏[2] 李晓东 冯爱玲 XU Rong;YANG Ye-Xin;LIN Min;LI Xiao-Dong;FENG Ai-Ling(School of Physics and Optoelectronics Technology,Baoji University of Arts and Sciences,Baoji 721016,China;The Key Laboratory of Biomedical Information Engineering of Ministry of Education,School of Life Science and Technology,Xi′an Jiaotong University,Xi′an 710049,China)

机构地区：[1]宝鸡文理学院物理与光电技术学院,宝鸡721016 [2]西安交通大学生命科学与技术学院生物信息工程教育部重点实验室,西安710049

出　　处：《分析化学》2020年第9期1202-1209,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.51801001);陕西省重点研发计划(No.2019GY-197);陕西省自然科学基础研究计划(No.2015JM5215);中国博士后科学基金面上项目(No.2016M601878);宝鸡市科技计划项目(No.16RKX1-29)资助。

摘　　要：硝酸盐、亚硝酸盐和氨氮(简称“三氮”)是水体中的主要污染物,目前我国的地表水和浅层地下水中普遍存在三氮污染问题。本研究将显色法与纸基微流控技术结合,制备了单通道纸基微流控芯片,在优化的反应条件下,分别对3种含氮化合物进行检测,亚硝酸盐、硝酸盐和氨氮的检出限分别为0.001 mmol/L、1 mmol/L和1 mg/L;在实际样本的检测中,3种样本的加标回收率均在95.4%~114.9%之内。其次,通过合理的结构设计将分别用于3种含氮化合物检测的单通道纸基微流控芯片集成于一体,制备出用于三氮同时检测的多通道纸基微流控芯片,多通道纸基微流控芯片的检测结果与单通道纸基微流控芯片的检测结果具有良好的一致性。本研究提出的基于显色法的多通道纸基微流控芯片可对水中的三氮进行同时检测,并且,此芯片具有成本低廉、灵敏度高和便于操作等特点,适用于现场快速检测,有望在资源匮乏的水源污染地区推广。Nitrate,nitrite and ammonia nitrogen(3-Nitrogen)are the main pollutants in water.“3-Nitrogen”pollution appears in both surface water and shallow groundwater in China.In this study,we combined the chromogenic method with the microfluidic technology to prepare the chip for simultaneous detection of“3-Nitrogen”.Firstly,single-channel paper-based microfluidic chips were fabricated to verify the validity of the detection methods for three nitrogen-containing compounds and to optimize the reaction conditions.The corresponding detection limits were calculated to be 0.001 mmol/L,1 mmol/L and 1 mg/L,respectively.In the testing of the actual samples,the spiked recoveries of the three samples were 95.4%-114.9%.Secondly,through a reasonable structural design,the single-channel paper-based microfluidic chips for the detection of three nitrogen-containing compounds were integrated into a multi-channel paper-based microfluidic chip for simultaneous detection of“3-Nitrogen”.The detection results obtained by the multi-channel paper-based microfluidic chip maintained good consistency with those of the single-channel paper-based microfluidic chip.The multi-channel paper-based microfluidic chip based on chromogenic method proposed here could simultaneously detect“3-Nitrogen”in water,and the chip had the characteristics of low cost,high sensitivity and easy operation,as well as rapid detection.The technology showed great potential in detection of pollutants in water-contaminated areas with limited resources.

关 键 词：显色法 三氮检测 纸基微流控 

分 类 号：X832[环境科学与工程—环境工程]