直喷离子化质谱法快速鉴别粉防己和广防己  

Rapid Differentiation of Stephania tetrandra and Aristolochia fangchi by Direct Spray Mass Spectrometry

作　　者：张朝辉 陈应庄[1] 陈波 马铭[1] ZHANG Zhao-Hui;CHEN Ying-Zhuang;CHEN Bo;MA Ming(Key Laboratory of Chemical Biology&Traditional Chinese Medicine Research(Ministry of Education of China)and Key Laboratory of Phytochemical R&D of Hunan Province,Hunan Normal University,Changsha 410081,China;Hunan Biological and Electromechanical Polytechnic,Changsha 410127,China)

机构地区：[1]化学生物学及中药分析教育部重点实验室,植化单体开发与利用湖南省重点实验室,湖南师范大学,长沙410081 [2]湖南生物机电职业技术学院,长沙410127

出　　处：《分析化学》2020年第9期1236-1243,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21775041,21775040)资助。

摘　　要：采用直喷离子化质谱法,通过对粉防己中的粉防己碱和防己诺林碱、广防己中的马兜铃酸A和木兰花碱的敞开式质谱行为进行研究,获得了粉防己(Stephania tetrandra)及其有毒伪品广防己(Aristolochia fangchi)的敞开式质谱轮廓,建立了直喷离子化质谱法简单快速鉴别粉防己和广防己的方法。结果表明,在正离子模式下,从敞开式质谱轮廓上,可以利用粉防己的标志性成分粉防己碱和防己诺林碱对粉防已和广防己进行快速鉴别。此方法具有简单、快速、无需样品前处理等优点,对防己药材的质量控制具有重要参考价值。研究了马兜铃酸A在敞开式质谱条件下的电喷雾电离行为,对敞开式质谱检测技术分析马兜铃酸A有借鉴意义。The ambient mass spectrometry(MS)profiles of Stephania tetrandra and its toxic adulteration,Aristolochia fangchi,were obtained by using direct spray mass spectrometry.The MS behaviors of tetrandrine and fangchinoline in Stephania tetrandra,aristolochic acid A and magnoflorine in Aristolochia fangchi were studied.A simple and rapid method for distinguishing Stephania tetrandra from Aristolochia fangchi was established by analyzing their ambient MS profiles.The results showed that under the positive ion mode,Stephania tetrandra and Aristolochia fangchi could be identified easily and effectively according to their symbolic components,fangchinoline and tetrandrine in Stephania tetrandra.The method was simple,fast,effective,with no need of sample pretreatment,which was helpful for the quality control of fangchi herbal medicine.At the same time,the study on the electrospray behavior of aristolochic acid A has certain reference significance for the ambient MS analysis of aristolochic acid A.

关 键 词：直喷离子化质谱法 粉防己 广防己 防己诺林碱 粉防己碱 木兰花碱 马兜铃酸A 

分 类 号：R282.5[医药卫生—中药学] O657.63[医药卫生—中医学]