COVID-19患者外周血T淋巴细胞计数动态变化研究  

Dynamic changes of peripheral blood T lymphocytes in COVID-19 patients

作　　者：陈婉玲 王伟峰 李文涛[2] 陈逢梅[1] 高碧华[1] 龙凤 曾厚洋 吕建楠 Chen Wanling;Wang Weifeng;Li Wentao;Chen Fengmei;Gao Bihua;Long Feng;Zeng Houyang;Lyu Jiannan(Department of Infectious Diseases,Beihai People′s Hospital,Beihai 536000,China;Department of Respiratory and Critical Care Medicine,the Second Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西北海市人民医院感染性疾病科,536000 [2]广西医科大学第二附属医院呼吸与危重症医学科,南宁530021

出　　处：《中华微生物学和免疫学杂志》2020年第7期495-498,共4页Chinese Journal of Microbiology and Immunology

基　　金：北海市科技计划项目(北科合202082060)。

摘　　要：目的探讨COVID-19患者T淋巴细胞的动态变化。方法收集2020年1—2月在北海市人民医院感染性疾病科确诊为COVID-19的40例患者和同期40名健康体检者(健康对照组)的抗凝血,流式细胞仪检测CD4+T和CD8+T淋巴细胞亚群计数。同时检测24例连续2次核酸检测阴性达到出院标准的恢复期患者CD4+T和CD8+T淋巴细胞亚群计数。结果与健康对照组比,急性期COVID-19患者的白细胞及淋巴细胞计数均显著降低[(4.71±1.54)×109个/L vs(6.26±1.44)×109个/L,(1.13±0.41)×109个/L vs(1.51±0.39)×109个/L;P均<0.05];CD4+T和CD8+T淋巴细胞数量均显著减少[(447.15±144.42)个/μl vs(592.83±146.76)个/μl,(309.35±173.05)个/μl vs(397.20±136.94)个/μl;P均<0.05],而CD4+/CD8+T细胞比例与健康对照组比差异无统计学意义(P>0.05)。24例出院患者恢复期的CD4+T和CD8+T淋巴细胞数量较急性期显著升高[(598.08±138.71)个/μl vs(420.67±147.38)个/μl,(439.08±166.94)个/μl vs(296.67±151.06)个/μl;P均<0.05],与健康对照组比差异无统计学意义(P>0.05)。结论急性期COVID-19患者的免疫功能出现一过性免疫缺陷,在恢复期可升高至正常水平。Objective To analyze the dynamic changes of T lymphocytes in patients with COVID-19.Methods Blood samples were collected from 40 COVID-19 cases and 40 healthy controls in Beihai People′s Hospital from January to February,2020.The counts of CD4+T and CD8+T lymphocytes were detected by flow cytometry.Moreover,the T lymphocyte counts in 24 convalescent patients with two consecutive negative nucleic acid test results were also detected.Results The leukocytes and lymphocytes in the patients with acute COVID-19 were significantly lower than those in the healthy controls[(4.71±1.54)×109 cell/L vs(6.26±1.44)×109 cell/L,(1.13±0.41)×109 cell/L vs(1.51±0.39)×109 cell/L;both P<0.05].The counts of CD4+T and CD8+T lymphocytes in the patients with acute COVID-19 were significantly lower than those in the healthy controls[(447.15±144.42)cell/μl vs(592.83±146.76)cell/μl,(309.35±173.05)cell/μl vs(397.20±136.94)cell/μl;both P<0.05],while no significant difference was observed in the CD4+/CD8+T cell ratio(P>0.05).In the 24 convalescent COVID-19 patients,the counts of CD4+T and CD8+T lymphocytes were higher during convalescence than in the acute phase[(598.08±138.71)cell/μl vs(420.67±147.38)cell/μl,(439.08±166.94)cell/μl vs(296.67±151.06)cell/μl;both P<0.05],but there was no significant difference in the T lymphocyte counts between the convalescent patients and the healthy controls(P>0.05).Conclusions A transient immune deficiency occurred in patients with acute COVID-19,but the impaired immune function could restore to normal level during recovery.

关 键 词：COVID-19 CD4 CD8 T淋巴细胞 

分 类 号：R563.1[医药卫生—呼吸系统]