A high-quality genome sequence of alkaligrass provides insights into halophyte stress tolerance  

作　　者：Wenting Zhang Jie Liu Yongxue Zhang Jie Qiu Ying Li Baojiang Zheng Fenhong Hu Shaojun Dai Xuehui Huang 

机构地区：[1]Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China [2]Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University),Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040,China

出　　处：《Science China(Life Sciences)》2020年第9期1269-1282,共14页中国科学（生命科学英文版）

基　　金：supported by grants from the National Key Research and Development Program of China(2018YFA090060);the Foundation of Shanghai Science and Technology Committee(17391900600);the Fund of Shanghai Engineering Research Center of Plant Germplasm Resources(17DZ2252700);the Natural Science Foundation of Heilongjiang Province(ZD2019C003)。

摘　　要：Alkaligrass(Puccinellia tenuiflora) is a monocotyledonous halophytic forage grass widely distributed in Northern China. It belongs to the Gramineae family and shares a close phylogenetic relationship with the cereal crops, wheat and barley. Here, we present a high-quality chromosome-level genome sequence of alkaligrass assembled from Illumina, Pac Bio and 10× Genomics reads combined with genome-wide chromosome conformation capture(Hi-C) data. The ~1.50 Gb assembled alkaligrass genome encodes 38,387 protein-coding genes, and 54.9% of the assembly are transposable elements, with long terminal repeats being the most abundant. Comparative genomic analysis coupled with stress-treated transcriptome profiling uncovers a set of unique saline-and alkaline-responsive genes in alkaligrass. The high-quality genome assembly and the identified stress related genes in alkaligrass provide an important resource for evolutionary genomic studies in Gramineae and facilitate further understanding of molecular mechanisms underlying stress tolerance in monocotyledonous halophytes. The alkaligrass genome data is freely available at http://xhhuanglab.cn/data/alkaligrass.html.

关 键 词：alkaligrass Puccinellia tenuiflora genome assembly saline-alkaline tolerance 

分 类 号：Q945.78[生物学—植物学]